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Transcriptomic regulations of heat stress response in the liver of lactating dairy cows

BACKGROUND: The global dairy industry is currently facing the challenge of heat stress (HS). Despite the implementation of various measures to mitigate the negative impact of HS on milk production, the cellular response of dairy cows to HS is still not well understood. Our study aims to analyze tran...

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Autores principales: Li, Guangsheng, Yu, Xingtan, Portela Fontoura, Ananda B., Javaid, Awais, de la Maza-Escolà, Víctor Sáinz, Salandy, Nia S., Fubini, Susan L., Grilli, Ester, McFadden, Joseph. W., Duan, Jingyue Ellie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10360291/
https://www.ncbi.nlm.nih.gov/pubmed/37474909
http://dx.doi.org/10.1186/s12864-023-09484-1
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author Li, Guangsheng
Yu, Xingtan
Portela Fontoura, Ananda B.
Javaid, Awais
de la Maza-Escolà, Víctor Sáinz
Salandy, Nia S.
Fubini, Susan L.
Grilli, Ester
McFadden, Joseph. W.
Duan, Jingyue Ellie
author_facet Li, Guangsheng
Yu, Xingtan
Portela Fontoura, Ananda B.
Javaid, Awais
de la Maza-Escolà, Víctor Sáinz
Salandy, Nia S.
Fubini, Susan L.
Grilli, Ester
McFadden, Joseph. W.
Duan, Jingyue Ellie
author_sort Li, Guangsheng
collection PubMed
description BACKGROUND: The global dairy industry is currently facing the challenge of heat stress (HS). Despite the implementation of various measures to mitigate the negative impact of HS on milk production, the cellular response of dairy cows to HS is still not well understood. Our study aims to analyze transcriptomic dynamics and functional changes in the liver of cows subjected to heat stress (HS). To achieve this, a total of 9 Holstein dairy cows were randomly selected from three environmental conditions - heat stress (HS), pair-fed (PF), and thermoneutral (TN) groups - and liver biopsies were obtained for transcriptome analysis. RESULTS: Both the dry matter intake (DMI) and milk yield of cows in the HS group exhibited significant reduction compared to the TN group. Through liver transcriptomic analysis, 483 differentially expressed genes (DEGs) were identified among three experimental groups. Especially, we found all the protein coding genes in mitochondria were significantly downregulated under HS and 6 heat shock proteins were significant upregulated after HS exposure, indicating HS may affect mitochondria integrity and jeopardize the metabolic homeostasis in liver. Furthermore, Gene ontology (GO) enrichment of DEGs revealed that the protein folding pathway was upregulated while oxidative phosphorylation was downregulated in the HS group, corresponding to impaired energy production caused by mitochondria dysfunction. CONCLUSIONS: The liver transcriptome analysis generated a comprehensive gene expression regulation network upon HS in lactating dairy cows. Overall, this study provides novel insights into molecular and metabolic changes of cows conditioned under HS. The key genes and pathways identified in this study provided further understanding of transcriptome regulation of HS response and could serve as vital references to mitigate the HS effects on dairy cow health and productivity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09484-1.
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spelling pubmed-103602912023-07-22 Transcriptomic regulations of heat stress response in the liver of lactating dairy cows Li, Guangsheng Yu, Xingtan Portela Fontoura, Ananda B. Javaid, Awais de la Maza-Escolà, Víctor Sáinz Salandy, Nia S. Fubini, Susan L. Grilli, Ester McFadden, Joseph. W. Duan, Jingyue Ellie BMC Genomics Research BACKGROUND: The global dairy industry is currently facing the challenge of heat stress (HS). Despite the implementation of various measures to mitigate the negative impact of HS on milk production, the cellular response of dairy cows to HS is still not well understood. Our study aims to analyze transcriptomic dynamics and functional changes in the liver of cows subjected to heat stress (HS). To achieve this, a total of 9 Holstein dairy cows were randomly selected from three environmental conditions - heat stress (HS), pair-fed (PF), and thermoneutral (TN) groups - and liver biopsies were obtained for transcriptome analysis. RESULTS: Both the dry matter intake (DMI) and milk yield of cows in the HS group exhibited significant reduction compared to the TN group. Through liver transcriptomic analysis, 483 differentially expressed genes (DEGs) were identified among three experimental groups. Especially, we found all the protein coding genes in mitochondria were significantly downregulated under HS and 6 heat shock proteins were significant upregulated after HS exposure, indicating HS may affect mitochondria integrity and jeopardize the metabolic homeostasis in liver. Furthermore, Gene ontology (GO) enrichment of DEGs revealed that the protein folding pathway was upregulated while oxidative phosphorylation was downregulated in the HS group, corresponding to impaired energy production caused by mitochondria dysfunction. CONCLUSIONS: The liver transcriptome analysis generated a comprehensive gene expression regulation network upon HS in lactating dairy cows. Overall, this study provides novel insights into molecular and metabolic changes of cows conditioned under HS. The key genes and pathways identified in this study provided further understanding of transcriptome regulation of HS response and could serve as vital references to mitigate the HS effects on dairy cow health and productivity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09484-1. BioMed Central 2023-07-20 /pmc/articles/PMC10360291/ /pubmed/37474909 http://dx.doi.org/10.1186/s12864-023-09484-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Guangsheng
Yu, Xingtan
Portela Fontoura, Ananda B.
Javaid, Awais
de la Maza-Escolà, Víctor Sáinz
Salandy, Nia S.
Fubini, Susan L.
Grilli, Ester
McFadden, Joseph. W.
Duan, Jingyue Ellie
Transcriptomic regulations of heat stress response in the liver of lactating dairy cows
title Transcriptomic regulations of heat stress response in the liver of lactating dairy cows
title_full Transcriptomic regulations of heat stress response in the liver of lactating dairy cows
title_fullStr Transcriptomic regulations of heat stress response in the liver of lactating dairy cows
title_full_unstemmed Transcriptomic regulations of heat stress response in the liver of lactating dairy cows
title_short Transcriptomic regulations of heat stress response in the liver of lactating dairy cows
title_sort transcriptomic regulations of heat stress response in the liver of lactating dairy cows
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10360291/
https://www.ncbi.nlm.nih.gov/pubmed/37474909
http://dx.doi.org/10.1186/s12864-023-09484-1
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