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Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow
SIGNIFICANCE: Multi-photon fluorescence recovery after photobleaching (MPFRAP) is a nonlinear microscopy technique used to measure the diffusion coefficient of fluorescently tagged molecules in solution. Previous MPFRAP fitting models calculate the diffusion coefficient in systems with diffusion or...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362154/ https://www.ncbi.nlm.nih.gov/pubmed/37484975 http://dx.doi.org/10.1117/1.JBO.28.7.076502 |