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Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow

SIGNIFICANCE: Multi-photon fluorescence recovery after photobleaching (MPFRAP) is a nonlinear microscopy technique used to measure the diffusion coefficient of fluorescently tagged molecules in solution. Previous MPFRAP fitting models calculate the diffusion coefficient in systems with diffusion or...

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Detalles Bibliográficos
Autores principales:	Elias, Tresa M., Brown, Edward B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Society of Photo-Optical Instrumentation Engineers 2023
Materias:	Microscopy
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362154/ https://www.ncbi.nlm.nih.gov/pubmed/37484975 http://dx.doi.org/10.1117/1.JBO.28.7.076502

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