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Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance
SIGNIFICANCE: India has one of the highest rates of oral squamous cell carcinoma (OSCC) in the world, with an incidence of 15 per 100,000 and more than 70,000 deaths per year. The problem is exacerbated by a lack of medical infrastructure and routine screening, especially in rural areas. New technol...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362156/ https://www.ncbi.nlm.nih.gov/pubmed/37483565 http://dx.doi.org/10.1117/1.JBO.28.8.082809 |
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author | Khan, Shakir Song, Bofan Mallidi, Srivalleesha Li, Shaobai Liu, Hui Bilal Hussain, M. A. Siddiqui, Shaista Khan, Amjad P. Akhtar, Kafil Siddiqui, Shahid Ali Hasan, Syed Abrar Hopper, Colin Bown, Stephen G. Liang, Rongguang Hasan, Tayyaba Celli, Jonathan P. |
author_facet | Khan, Shakir Song, Bofan Mallidi, Srivalleesha Li, Shaobai Liu, Hui Bilal Hussain, M. A. Siddiqui, Shaista Khan, Amjad P. Akhtar, Kafil Siddiqui, Shahid Ali Hasan, Syed Abrar Hopper, Colin Bown, Stephen G. Liang, Rongguang Hasan, Tayyaba Celli, Jonathan P. |
author_sort | Khan, Shakir |
collection | PubMed |
description | SIGNIFICANCE: India has one of the highest rates of oral squamous cell carcinoma (OSCC) in the world, with an incidence of 15 per 100,000 and more than 70,000 deaths per year. The problem is exacerbated by a lack of medical infrastructure and routine screening, especially in rural areas. New technologies for oral cancer detection and timely treatment at the point of care are urgently needed. AIM: Our study aimed to use a hand-held smartphone-coupled intraoral imaging device, previously investigated for autofluorescence (auto-FL) diagnostics adapted here for treatment guidance and monitoring photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence (FL). APPROACH: A total of 12 patients with 14 buccal mucosal lesions having moderately/well-differentiated micro-invasive OSCC lesions ([Formula: see text] diameter and [Formula: see text] depth) were systemically (in oral solution) administered three doses of [Formula: see text] ALA (total [Formula: see text]). Lesion site PpIX and auto-FL were imaged using the multichannel FL and polarized white-light oral cancer imaging probe before/after ALA administration and after light delivery (fractionated, total [Formula: see text] of 635 nm red LED light). RESULTS: The handheld device was conducive for access to lesion site images in the oral cavity. Segmentation of ratiometric images in which PpIX FL is mapped relative to auto-FL enabled improved demarcation of lesion boundaries relative to PpIX alone. A relative FL ([Formula: see text]-value) threshold of 1.4 was found to segment lesion site PpIX production among the patients with mild to severe dysplasia malignancy. The segmented lesion size is well correlated with ultrasound findings. Lesions for which [Formula: see text]-value was [Formula: see text] at the time of treatment were associated with successful outcomes. CONCLUSION: These results indicate the utility of a low-cost, handheld intraoral imaging probe for image-guided PDT and treatment monitoring while also laying the groundwork for an integrated approach, combining cancer screening and treatment with the same hardware. |
format | Online Article Text |
id | pubmed-10362156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-103621562023-07-23 Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance Khan, Shakir Song, Bofan Mallidi, Srivalleesha Li, Shaobai Liu, Hui Bilal Hussain, M. A. Siddiqui, Shaista Khan, Amjad P. Akhtar, Kafil Siddiqui, Shahid Ali Hasan, Syed Abrar Hopper, Colin Bown, Stephen G. Liang, Rongguang Hasan, Tayyaba Celli, Jonathan P. J Biomed Opt Special Section on Seeing Inside Tissue with Optical Molecular Probes SIGNIFICANCE: India has one of the highest rates of oral squamous cell carcinoma (OSCC) in the world, with an incidence of 15 per 100,000 and more than 70,000 deaths per year. The problem is exacerbated by a lack of medical infrastructure and routine screening, especially in rural areas. New technologies for oral cancer detection and timely treatment at the point of care are urgently needed. AIM: Our study aimed to use a hand-held smartphone-coupled intraoral imaging device, previously investigated for autofluorescence (auto-FL) diagnostics adapted here for treatment guidance and monitoring photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence (FL). APPROACH: A total of 12 patients with 14 buccal mucosal lesions having moderately/well-differentiated micro-invasive OSCC lesions ([Formula: see text] diameter and [Formula: see text] depth) were systemically (in oral solution) administered three doses of [Formula: see text] ALA (total [Formula: see text]). Lesion site PpIX and auto-FL were imaged using the multichannel FL and polarized white-light oral cancer imaging probe before/after ALA administration and after light delivery (fractionated, total [Formula: see text] of 635 nm red LED light). RESULTS: The handheld device was conducive for access to lesion site images in the oral cavity. Segmentation of ratiometric images in which PpIX FL is mapped relative to auto-FL enabled improved demarcation of lesion boundaries relative to PpIX alone. A relative FL ([Formula: see text]-value) threshold of 1.4 was found to segment lesion site PpIX production among the patients with mild to severe dysplasia malignancy. The segmented lesion size is well correlated with ultrasound findings. Lesions for which [Formula: see text]-value was [Formula: see text] at the time of treatment were associated with successful outcomes. CONCLUSION: These results indicate the utility of a low-cost, handheld intraoral imaging probe for image-guided PDT and treatment monitoring while also laying the groundwork for an integrated approach, combining cancer screening and treatment with the same hardware. Society of Photo-Optical Instrumentation Engineers 2023-07-21 2023-08 /pmc/articles/PMC10362156/ /pubmed/37483565 http://dx.doi.org/10.1117/1.JBO.28.8.082809 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Special Section on Seeing Inside Tissue with Optical Molecular Probes Khan, Shakir Song, Bofan Mallidi, Srivalleesha Li, Shaobai Liu, Hui Bilal Hussain, M. A. Siddiqui, Shaista Khan, Amjad P. Akhtar, Kafil Siddiqui, Shahid Ali Hasan, Syed Abrar Hopper, Colin Bown, Stephen G. Liang, Rongguang Hasan, Tayyaba Celli, Jonathan P. Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
title | Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
title_full | Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
title_fullStr | Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
title_full_unstemmed | Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
title_short | Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
title_sort | clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance |
topic | Special Section on Seeing Inside Tissue with Optical Molecular Probes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362156/ https://www.ncbi.nlm.nih.gov/pubmed/37483565 http://dx.doi.org/10.1117/1.JBO.28.8.082809 |
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