An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments

Large biosynthetic gene cluster (BGC) cloning is important for discovering natural product-based drugs and remains challenging in high GC content microorganisms (e.g., Actinobacteria). Here, we present an in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments. We describe...

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Detalles Bibliográficos
Autores principales: Zeng, Xiaoqian, Wang, Shuliu, Liang, Mindong, Wang, Weishan, Jiang, Yue, Xu, Fei, Liu, Leshi, Yan, Hao, Tong, Yaojun, Zhang, Lixin, Tan, Gao-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362190/
https://www.ncbi.nlm.nih.gov/pubmed/37432853
http://dx.doi.org/10.1016/j.xpro.2023.102435

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