Therapeutic effects of avocado (Persea americana Mill.) seed powder against reproductive toxicity induced by Mancozeb (herbicide) in female rabbits

Pesticides like Mancozeb are being increasingly indispensable in the control of crop pests. Unfortunately, they have been implicated in genotoxicity due to their ubiquity, toxicological properties, persistence and presence in the food chain. This study sought to evaluate the efficacy of powdered avocado seed on reproductive parameters in the management of oxidative stress in female rabbits caused by the herbicide Mancozeb. Twenty-eight female rabbits aged 7–8 months and weighing between 2780.4 g and 3143.7 g were randomly divided into four groups of seven rabbits each. Each group received for 90 consecutive days distilled water or Mancozeb associated or not with avocado seed powder orally as follows: T1: 10 ml distilled water; T2, T3 and T4: 100 mg/kg bw Mancozeb. This was followed by oral administration of 250, 500, and 0 mg/kg of avocado seed powder for T2, T3, and T4, respectively. Water and feed were distributed ad libitum. Collected data concerned growth, carcass and reproductive performances, hematological and biochemistry characteristics. Results demonstrated that pregnant and lactating female rabbits administered Mancozeb exhibited a significant decrease (P < 0.05) in food intake, body weight, and body weight gain. Female rabbits exposed to Mancozeb had a decrease in litter size and weight from birth to weaning, as well as in weaning body weight and weight increase, fertility and prolificacy rate, milk yield, and daily milk efficiency. However, administration of avocado seed powder reversed (P < 0.05) the trends in these parameters in a dose-dependent manner. The increase in relative weight of the kidney and liver, concentrations of urea, creatinine, alanine and aspartate aminotransferases, mean cell volume, white blood cells, and lymphocytes were all associated with increased Mancozeb rates (P < 0.05). On the contrary, administration of the Mancozeb caused decrease in hemoglobin (Hb), Red blood Cell (RBC) and protein content. Administration of avocado seed powder significantly (P < 0.05) ameliorated the Mancozeb effects on these parameters. Applying 500 mg/kg b.w Avocado seed powder may be suggested as an alternative therapy for reproductive defects induced by Mancozeb in female rabbits.