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miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14

Duck Tembusu virus (DTMUV), belonging to the Flaviviridae family, is a major virus that affects duck health in China. MicroRNAs (miRNAs) play an important role in viral replication. However, little is known about the function of miRNAs during DTMUV infection. Here, the host miR-146b-5p was found to...

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Autores principales: Huang, Juan, Lei, Lin, Cui, Min, Cheng, Anchun, Wang, Mingshu, Liu, Mafeng, Zhu, Dekang, Chen, Shun, Zhao, Xinxin, Yang, Qiao, Wu, Ying, Zhang, Shaqiu, Ou, Xumin, Mao, Sai, Gao, Qun, Sun, Di, Tian, Bin, Yin, Zhongqiong, Jia, Renyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362356/
https://www.ncbi.nlm.nih.gov/pubmed/37441905
http://dx.doi.org/10.1016/j.psj.2023.102890
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author Huang, Juan
Lei, Lin
Cui, Min
Cheng, Anchun
Wang, Mingshu
Liu, Mafeng
Zhu, Dekang
Chen, Shun
Zhao, Xinxin
Yang, Qiao
Wu, Ying
Zhang, Shaqiu
Ou, Xumin
Mao, Sai
Gao, Qun
Sun, Di
Tian, Bin
Yin, Zhongqiong
Jia, Renyong
author_facet Huang, Juan
Lei, Lin
Cui, Min
Cheng, Anchun
Wang, Mingshu
Liu, Mafeng
Zhu, Dekang
Chen, Shun
Zhao, Xinxin
Yang, Qiao
Wu, Ying
Zhang, Shaqiu
Ou, Xumin
Mao, Sai
Gao, Qun
Sun, Di
Tian, Bin
Yin, Zhongqiong
Jia, Renyong
author_sort Huang, Juan
collection PubMed
description Duck Tembusu virus (DTMUV), belonging to the Flaviviridae family, is a major virus that affects duck health in China. MicroRNAs (miRNAs) play an important role in viral replication. However, little is known about the function of miRNAs during DTMUV infection. Here, the host miR-146b-5p was found to regulate DTMUV replication. When DTMUV infected duck embryo fibroblasts (DEFs), the expression levels of miR-146b-5p increased significantly over time. Moreover, the viral RNA copies, E protein expression levels and virus titers were all upregulated when miR-146b-5p was overexpressed in DEFs. The opposite results were also observed upon knockdown of miR-146b-5p in DEFs. To explore the mechanism by which miR-146b-5p promoted DTMUV replication, mass spectrometry, and RNA pull-down assays were employed. Ribosomal protein S14 (RPS14), a component of 40S ribosomal proteins, was identified to interact with miR-146b-5p. In addition, the relative mRNA expression levels of RPS14 gene were negatively modulated by miR-146b-5p. Subsequently, it was found that overexpression of RPS14 could decrease the replication of DTMUV, and the reverse results were also detected by knockdown of RPS14. In conclusion, this study revealed that miR-146b-5p promoted DTMUV replication by targeting RPS14, which provides a new mechanism by which DTMUV evades host defenses and a new direction for further antiviral strategies development.
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spelling pubmed-103623562023-07-23 miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14 Huang, Juan Lei, Lin Cui, Min Cheng, Anchun Wang, Mingshu Liu, Mafeng Zhu, Dekang Chen, Shun Zhao, Xinxin Yang, Qiao Wu, Ying Zhang, Shaqiu Ou, Xumin Mao, Sai Gao, Qun Sun, Di Tian, Bin Yin, Zhongqiong Jia, Renyong Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Duck Tembusu virus (DTMUV), belonging to the Flaviviridae family, is a major virus that affects duck health in China. MicroRNAs (miRNAs) play an important role in viral replication. However, little is known about the function of miRNAs during DTMUV infection. Here, the host miR-146b-5p was found to regulate DTMUV replication. When DTMUV infected duck embryo fibroblasts (DEFs), the expression levels of miR-146b-5p increased significantly over time. Moreover, the viral RNA copies, E protein expression levels and virus titers were all upregulated when miR-146b-5p was overexpressed in DEFs. The opposite results were also observed upon knockdown of miR-146b-5p in DEFs. To explore the mechanism by which miR-146b-5p promoted DTMUV replication, mass spectrometry, and RNA pull-down assays were employed. Ribosomal protein S14 (RPS14), a component of 40S ribosomal proteins, was identified to interact with miR-146b-5p. In addition, the relative mRNA expression levels of RPS14 gene were negatively modulated by miR-146b-5p. Subsequently, it was found that overexpression of RPS14 could decrease the replication of DTMUV, and the reverse results were also detected by knockdown of RPS14. In conclusion, this study revealed that miR-146b-5p promoted DTMUV replication by targeting RPS14, which provides a new mechanism by which DTMUV evades host defenses and a new direction for further antiviral strategies development. Elsevier 2023-06-21 /pmc/articles/PMC10362356/ /pubmed/37441905 http://dx.doi.org/10.1016/j.psj.2023.102890 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Huang, Juan
Lei, Lin
Cui, Min
Cheng, Anchun
Wang, Mingshu
Liu, Mafeng
Zhu, Dekang
Chen, Shun
Zhao, Xinxin
Yang, Qiao
Wu, Ying
Zhang, Shaqiu
Ou, Xumin
Mao, Sai
Gao, Qun
Sun, Di
Tian, Bin
Yin, Zhongqiong
Jia, Renyong
miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14
title miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14
title_full miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14
title_fullStr miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14
title_full_unstemmed miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14
title_short miR-146b-5p promotes duck Tembusu virus replication by targeting RPS14
title_sort mir-146b-5p promotes duck tembusu virus replication by targeting rps14
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362356/
https://www.ncbi.nlm.nih.gov/pubmed/37441905
http://dx.doi.org/10.1016/j.psj.2023.102890
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