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Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties

The common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learn the rhizobial communities associated with the common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (...

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Autores principales: Wang, Ziqi, Liu, Lili, Hu, Dong, Wang, En Tao, Gu, Chuntao, Wang, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362387/
https://www.ncbi.nlm.nih.gov/pubmed/37485523
http://dx.doi.org/10.3389/fmicb.2023.1195307
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author Wang, Ziqi
Liu, Lili
Hu, Dong
Wang, En Tao
Gu, Chuntao
Wang, Hao
author_facet Wang, Ziqi
Liu, Lili
Hu, Dong
Wang, En Tao
Gu, Chuntao
Wang, Hao
author_sort Wang, Ziqi
collection PubMed
description The common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learn the rhizobial communities associated with the common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (Cuican and Jiadouwang) grown in two sites of blackland and were characterized by comparative sequence analyses of 16S rRNA, recA, atpD, nodC, and nifH genes, and whole genome. As a result, Rhizobium indigoferae, R. anhuiense, and R. croatiense as minor groups and three dominant novel Rhizobium species were identified based on their average nucleotide identity and DNA–DNA hybridization values to the type strains of relative species. This community composition of rhizobia associated with the common bean in the tested black soils was unique. Despite their different species affiliations, all of them were identified into the symbiovar phaseoli according to the phylogenies of symbiotic genes, nodC and nifH. While the phylogenetic discrepancies found in nodC, nifH evidenced that the evolutions of nodulation (nod) and nitrogen fixation (nif ) genes were partially independent. In addition, only one dominant rhizobial species was shared by the two common bean varieties grown in the two soil samples, implying that both the plant variety and the soil characteristics affected the compatibility between rhizobia and their hosts. These findings further enlarged the spectrum of common bean-nodulating rhizobia and added more information about the interactions among the soil factors, rhizobial species, and host plants in the symbiosis.
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spelling pubmed-103623872023-07-23 Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties Wang, Ziqi Liu, Lili Hu, Dong Wang, En Tao Gu, Chuntao Wang, Hao Front Microbiol Microbiology The common bean (Phaseolus vulgaris L.) is an important crop in the world that forms root nodules with diverse rhizobia. Aiming to learn the rhizobial communities associated with the common bean in the black soil of Northeast China, 79 rhizobia were isolated from root nodules of two host varieties (Cuican and Jiadouwang) grown in two sites of blackland and were characterized by comparative sequence analyses of 16S rRNA, recA, atpD, nodC, and nifH genes, and whole genome. As a result, Rhizobium indigoferae, R. anhuiense, and R. croatiense as minor groups and three dominant novel Rhizobium species were identified based on their average nucleotide identity and DNA–DNA hybridization values to the type strains of relative species. This community composition of rhizobia associated with the common bean in the tested black soils was unique. Despite their different species affiliations, all of them were identified into the symbiovar phaseoli according to the phylogenies of symbiotic genes, nodC and nifH. While the phylogenetic discrepancies found in nodC, nifH evidenced that the evolutions of nodulation (nod) and nitrogen fixation (nif ) genes were partially independent. In addition, only one dominant rhizobial species was shared by the two common bean varieties grown in the two soil samples, implying that both the plant variety and the soil characteristics affected the compatibility between rhizobia and their hosts. These findings further enlarged the spectrum of common bean-nodulating rhizobia and added more information about the interactions among the soil factors, rhizobial species, and host plants in the symbiosis. Frontiers Media S.A. 2023-07-06 /pmc/articles/PMC10362387/ /pubmed/37485523 http://dx.doi.org/10.3389/fmicb.2023.1195307 Text en Copyright © 2023 Wang, Liu, Hu, Wang, Gu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Ziqi
Liu, Lili
Hu, Dong
Wang, En Tao
Gu, Chuntao
Wang, Hao
Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties
title Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties
title_full Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties
title_fullStr Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties
title_full_unstemmed Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties
title_short Diversity of common bean rhizobia in blackland of northeastern China and their symbiotic compatibility with two host varieties
title_sort diversity of common bean rhizobia in blackland of northeastern china and their symbiotic compatibility with two host varieties
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10362387/
https://www.ncbi.nlm.nih.gov/pubmed/37485523
http://dx.doi.org/10.3389/fmicb.2023.1195307
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