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Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth
Newts have the extraordinary ability to fully regenerate lost or damaged cardiac, neural and retinal tissues, and even amputated limbs. In contrast, mammals lack these broad regenerative capabilities. While the molecular basis of newts’ regenerative ability is the subject of active study, the underl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10363125/ https://www.ncbi.nlm.nih.gov/pubmed/37481602 http://dx.doi.org/10.1038/s41598-023-38671-z |
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author | Middleton, Ryan C. Liao, Ke Liu, Weixin de Couto, Geoff Garcia, Nahuel Antes, Travis Wang, Yizhou Wu, Di Li, Xinling Tourtellotte, Warren G. Marbán, Eduardo |
author_facet | Middleton, Ryan C. Liao, Ke Liu, Weixin de Couto, Geoff Garcia, Nahuel Antes, Travis Wang, Yizhou Wu, Di Li, Xinling Tourtellotte, Warren G. Marbán, Eduardo |
author_sort | Middleton, Ryan C. |
collection | PubMed |
description | Newts have the extraordinary ability to fully regenerate lost or damaged cardiac, neural and retinal tissues, and even amputated limbs. In contrast, mammals lack these broad regenerative capabilities. While the molecular basis of newts’ regenerative ability is the subject of active study, the underlying paracrine signaling factors involved remain largely uncharacterized. Extracellular vesicles (EVs) play an important role in cell-to-cell communication via EV cargo-mediated regulation of gene expression patterns within the recipient cells. Here, we report that newt myogenic precursor (A1) cells secrete EVs (A1EVs) that contain messenger RNAs associated with early embryonic development, neuronal differentiation, and cell survival. Exposure of rat primary superior cervical ganglion (SCG) neurons to A1EVs increased neurite outgrowth, facilitated by increases in mitochondrial respiration. Canonical pathway analysis pinpointed activation of NGF/ERK5 signaling in SCG neurons exposed to A1EV, which was validated experimentally. Thus, newt EVs drive neurite growth and complexity in mammalian primary neurons. |
format | Online Article Text |
id | pubmed-10363125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103631252023-07-24 Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth Middleton, Ryan C. Liao, Ke Liu, Weixin de Couto, Geoff Garcia, Nahuel Antes, Travis Wang, Yizhou Wu, Di Li, Xinling Tourtellotte, Warren G. Marbán, Eduardo Sci Rep Article Newts have the extraordinary ability to fully regenerate lost or damaged cardiac, neural and retinal tissues, and even amputated limbs. In contrast, mammals lack these broad regenerative capabilities. While the molecular basis of newts’ regenerative ability is the subject of active study, the underlying paracrine signaling factors involved remain largely uncharacterized. Extracellular vesicles (EVs) play an important role in cell-to-cell communication via EV cargo-mediated regulation of gene expression patterns within the recipient cells. Here, we report that newt myogenic precursor (A1) cells secrete EVs (A1EVs) that contain messenger RNAs associated with early embryonic development, neuronal differentiation, and cell survival. Exposure of rat primary superior cervical ganglion (SCG) neurons to A1EVs increased neurite outgrowth, facilitated by increases in mitochondrial respiration. Canonical pathway analysis pinpointed activation of NGF/ERK5 signaling in SCG neurons exposed to A1EV, which was validated experimentally. Thus, newt EVs drive neurite growth and complexity in mammalian primary neurons. Nature Publishing Group UK 2023-07-22 /pmc/articles/PMC10363125/ /pubmed/37481602 http://dx.doi.org/10.1038/s41598-023-38671-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Middleton, Ryan C. Liao, Ke Liu, Weixin de Couto, Geoff Garcia, Nahuel Antes, Travis Wang, Yizhou Wu, Di Li, Xinling Tourtellotte, Warren G. Marbán, Eduardo Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth |
title | Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth |
title_full | Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth |
title_fullStr | Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth |
title_full_unstemmed | Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth |
title_short | Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth |
title_sort | newt a1 cell-derived extracellular vesicles promote mammalian nerve growth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10363125/ https://www.ncbi.nlm.nih.gov/pubmed/37481602 http://dx.doi.org/10.1038/s41598-023-38671-z |
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