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Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives

BACKGROUND: Analysis of the glutamine metabolic pathway has taken a special place in metabolomics research in recent years, given its important role in cell biosynthesis and bioenergetics across several disorders, especially in cancer cell survival. The science of metabolomics addresses the intricat...

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Autores principales: Mirveis, Zohreh, Howe, Orla, Cahill, Paul, Patil, Nitin, Byrne, Hugh J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10363518/
https://www.ncbi.nlm.nih.gov/pubmed/37482587
http://dx.doi.org/10.1007/s11306-023-02031-9
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author Mirveis, Zohreh
Howe, Orla
Cahill, Paul
Patil, Nitin
Byrne, Hugh J.
author_facet Mirveis, Zohreh
Howe, Orla
Cahill, Paul
Patil, Nitin
Byrne, Hugh J.
author_sort Mirveis, Zohreh
collection PubMed
description BACKGROUND: Analysis of the glutamine metabolic pathway has taken a special place in metabolomics research in recent years, given its important role in cell biosynthesis and bioenergetics across several disorders, especially in cancer cell survival. The science of metabolomics addresses the intricate intracellular metabolic network by exploring and understanding how cells function and respond to external or internal perturbations to identify potential therapeutic targets. However, despite recent advances in metabolomics, monitoring the kinetics of a metabolic pathway in a living cell in situ, real-time and holistically remains a significant challenge. AIM: This review paper explores the range of analytical approaches for monitoring metabolic pathways, as well as physicochemical modeling techniques, with a focus on glutamine metabolism. We discuss the advantages and disadvantages of each method and explore the potential of label-free Raman microspectroscopy, in conjunction with kinetic modeling, to enable real-time and in situ monitoring of the cellular kinetics of the glutamine metabolic pathway. KEY SCIENTIFIC CONCEPTS: Given its important role in cell metabolism, the ability to monitor and model the glutamine metabolic pathways are highlighted. Novel, label free approaches have the potential to revolutionise metabolic biosensing, laying the foundation for a new paradigm in metabolomics research and addressing the challenges in monitoring metabolic pathways in living cells. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s11306-023-02031-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-103635182023-07-25 Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives Mirveis, Zohreh Howe, Orla Cahill, Paul Patil, Nitin Byrne, Hugh J. Metabolomics Review Article BACKGROUND: Analysis of the glutamine metabolic pathway has taken a special place in metabolomics research in recent years, given its important role in cell biosynthesis and bioenergetics across several disorders, especially in cancer cell survival. The science of metabolomics addresses the intricate intracellular metabolic network by exploring and understanding how cells function and respond to external or internal perturbations to identify potential therapeutic targets. However, despite recent advances in metabolomics, monitoring the kinetics of a metabolic pathway in a living cell in situ, real-time and holistically remains a significant challenge. AIM: This review paper explores the range of analytical approaches for monitoring metabolic pathways, as well as physicochemical modeling techniques, with a focus on glutamine metabolism. We discuss the advantages and disadvantages of each method and explore the potential of label-free Raman microspectroscopy, in conjunction with kinetic modeling, to enable real-time and in situ monitoring of the cellular kinetics of the glutamine metabolic pathway. KEY SCIENTIFIC CONCEPTS: Given its important role in cell metabolism, the ability to monitor and model the glutamine metabolic pathways are highlighted. Novel, label free approaches have the potential to revolutionise metabolic biosensing, laying the foundation for a new paradigm in metabolomics research and addressing the challenges in monitoring metabolic pathways in living cells. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s11306-023-02031-9) contains supplementary material, which is available to authorized users. Springer US 2023-07-23 2023 /pmc/articles/PMC10363518/ /pubmed/37482587 http://dx.doi.org/10.1007/s11306-023-02031-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Article
Mirveis, Zohreh
Howe, Orla
Cahill, Paul
Patil, Nitin
Byrne, Hugh J.
Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
title Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
title_full Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
title_fullStr Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
title_full_unstemmed Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
title_short Monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
title_sort monitoring and modelling the glutamine metabolic pathway: a review and future perspectives
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10363518/
https://www.ncbi.nlm.nih.gov/pubmed/37482587
http://dx.doi.org/10.1007/s11306-023-02031-9
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