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MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1

BACKGROUND: Skeletal muscle is the largest tissue in the body, and it affects motion, metabolism and homeostasis. Skeletal muscle development comprises myoblast proliferation, fusion and differentiation to form myotubes, which subsequently form mature muscle fibres. This process is strictly regulate...

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Autores principales: Cao, Haigang, Du, Tianning, Li, Chenchen, Wu, Lingling, Liu, Jieming, Guo, Yuan, Li, Xiao, Yang, Gongshe, Jin, Jianjun, Shi, Xin’e
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10364376/
https://www.ncbi.nlm.nih.gov/pubmed/37488537
http://dx.doi.org/10.1186/s12864-023-09431-0
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author Cao, Haigang
Du, Tianning
Li, Chenchen
Wu, Lingling
Liu, Jieming
Guo, Yuan
Li, Xiao
Yang, Gongshe
Jin, Jianjun
Shi, Xin’e
author_facet Cao, Haigang
Du, Tianning
Li, Chenchen
Wu, Lingling
Liu, Jieming
Guo, Yuan
Li, Xiao
Yang, Gongshe
Jin, Jianjun
Shi, Xin’e
author_sort Cao, Haigang
collection PubMed
description BACKGROUND: Skeletal muscle is the largest tissue in the body, and it affects motion, metabolism and homeostasis. Skeletal muscle development comprises myoblast proliferation, fusion and differentiation to form myotubes, which subsequently form mature muscle fibres. This process is strictly regulated by a series of molecular networks. Increasing evidence has shown that noncoding RNAs, especially microRNAs (miRNAs), play vital roles in regulating skeletal muscle growth. Here, we showed that miR-668-3p is highly expressed in skeletal muscle. METHODS: Proliferating and differentiated C2C12 cells were transfected with miR-668-3p mimics and/or inhibitor, and the mRNA and protein levels of its target gene were evaluated by RT‒qPCR and Western blotting analysis. The targeting of Appl1 by miR-668-3p was confirmed by dual luciferase assay. The interdependence of miR-668-3p and Appl1 was verified by cotransfection of C2C12 cells. RESULTS: Our data reveal that miR-668-3p can inhibit myoblast proliferation and myogenic differentiation. Phosphotyrosine interacting with PH domain and leucine zipper 1 (Appl1) is a target gene of miR-668-3p, and it can promote myoblast proliferation and differentiation by activating the p38 MAPK pathway. Furthermore, the inhibitory effect of miR-668-3p on myoblast cell proliferation and myogenic differentiation could be rescued by Appl1. CONCLUSION: Our results indicate a new mechanism by which the miR-668-3p/Appl1/p38 MAPK pathway regulates skeletal muscle development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09431-0.
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spelling pubmed-103643762023-07-25 MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1 Cao, Haigang Du, Tianning Li, Chenchen Wu, Lingling Liu, Jieming Guo, Yuan Li, Xiao Yang, Gongshe Jin, Jianjun Shi, Xin’e BMC Genomics Research BACKGROUND: Skeletal muscle is the largest tissue in the body, and it affects motion, metabolism and homeostasis. Skeletal muscle development comprises myoblast proliferation, fusion and differentiation to form myotubes, which subsequently form mature muscle fibres. This process is strictly regulated by a series of molecular networks. Increasing evidence has shown that noncoding RNAs, especially microRNAs (miRNAs), play vital roles in regulating skeletal muscle growth. Here, we showed that miR-668-3p is highly expressed in skeletal muscle. METHODS: Proliferating and differentiated C2C12 cells were transfected with miR-668-3p mimics and/or inhibitor, and the mRNA and protein levels of its target gene were evaluated by RT‒qPCR and Western blotting analysis. The targeting of Appl1 by miR-668-3p was confirmed by dual luciferase assay. The interdependence of miR-668-3p and Appl1 was verified by cotransfection of C2C12 cells. RESULTS: Our data reveal that miR-668-3p can inhibit myoblast proliferation and myogenic differentiation. Phosphotyrosine interacting with PH domain and leucine zipper 1 (Appl1) is a target gene of miR-668-3p, and it can promote myoblast proliferation and differentiation by activating the p38 MAPK pathway. Furthermore, the inhibitory effect of miR-668-3p on myoblast cell proliferation and myogenic differentiation could be rescued by Appl1. CONCLUSION: Our results indicate a new mechanism by which the miR-668-3p/Appl1/p38 MAPK pathway regulates skeletal muscle development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09431-0. BioMed Central 2023-07-24 /pmc/articles/PMC10364376/ /pubmed/37488537 http://dx.doi.org/10.1186/s12864-023-09431-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Cao, Haigang
Du, Tianning
Li, Chenchen
Wu, Lingling
Liu, Jieming
Guo, Yuan
Li, Xiao
Yang, Gongshe
Jin, Jianjun
Shi, Xin’e
MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
title MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
title_full MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
title_fullStr MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
title_full_unstemmed MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
title_short MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1
title_sort microrna-668-3p inhibits myoblast proliferation and differentiation by targeting appl1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10364376/
https://www.ncbi.nlm.nih.gov/pubmed/37488537
http://dx.doi.org/10.1186/s12864-023-09431-0
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