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Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

BACKGROUND: Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subsequently o...

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Detalles Bibliográficos
Autores principales:	Boselli, Daniela, Panigada, Maddalena, Di Terlizzi, Simona, Romanò, Monica, Canonico, Emanuele, Villa, Chiara, Minici, Claudia, van Anken, Eelco, Soprana, Elisa, Siccardi, Antonio G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10364397/ https://www.ncbi.nlm.nih.gov/pubmed/37482614 http://dx.doi.org/10.1186/s12967-023-04353-7

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