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Multiplexed microfluidic screening of bacterial chemotaxis

Microorganism sensing of and responding to ambient chemical gradients regulates a myriad of microbial processes that are fundamental to ecosystem function and human health and disease. The development of efficient, high-throughput screening tools for microbial chemotaxis is essential to disentanglin...

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Autores principales: Stehnach, Michael R, Henshaw, Richard J, Floge, Sheri A, Guasto, Jeffrey S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365836/
https://www.ncbi.nlm.nih.gov/pubmed/37486823
http://dx.doi.org/10.7554/eLife.85348
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author Stehnach, Michael R
Henshaw, Richard J
Floge, Sheri A
Guasto, Jeffrey S
author_facet Stehnach, Michael R
Henshaw, Richard J
Floge, Sheri A
Guasto, Jeffrey S
author_sort Stehnach, Michael R
collection PubMed
description Microorganism sensing of and responding to ambient chemical gradients regulates a myriad of microbial processes that are fundamental to ecosystem function and human health and disease. The development of efficient, high-throughput screening tools for microbial chemotaxis is essential to disentangling the roles of diverse chemical compounds and concentrations that control cell nutrient uptake, chemorepulsion from toxins, and microbial pathogenesis. Here, we present a novel microfluidic multiplexed chemotaxis device (MCD) which uses serial dilution to simultaneously perform six parallel bacterial chemotaxis assays that span five orders of magnitude in chemostimulant concentration on a single chip. We first validated the dilution and gradient generation performance of the MCD, and then compared the measured chemotactic response of an established bacterial chemotaxis system (Vibrio alginolyticus) to a standard microfluidic assay. Next, the MCD’s versatility was assessed by quantifying the chemotactic responses of different bacteria (Psuedoalteromonas haloplanktis, Escherichia coli) to different chemoattractants and chemorepellents. The MCD vastly accelerates the chemotactic screening process, which is critical to deciphering the complex sea of chemical stimuli underlying microbial responses.
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spelling pubmed-103658362023-07-25 Multiplexed microfluidic screening of bacterial chemotaxis Stehnach, Michael R Henshaw, Richard J Floge, Sheri A Guasto, Jeffrey S eLife Ecology Microorganism sensing of and responding to ambient chemical gradients regulates a myriad of microbial processes that are fundamental to ecosystem function and human health and disease. The development of efficient, high-throughput screening tools for microbial chemotaxis is essential to disentangling the roles of diverse chemical compounds and concentrations that control cell nutrient uptake, chemorepulsion from toxins, and microbial pathogenesis. Here, we present a novel microfluidic multiplexed chemotaxis device (MCD) which uses serial dilution to simultaneously perform six parallel bacterial chemotaxis assays that span five orders of magnitude in chemostimulant concentration on a single chip. We first validated the dilution and gradient generation performance of the MCD, and then compared the measured chemotactic response of an established bacterial chemotaxis system (Vibrio alginolyticus) to a standard microfluidic assay. Next, the MCD’s versatility was assessed by quantifying the chemotactic responses of different bacteria (Psuedoalteromonas haloplanktis, Escherichia coli) to different chemoattractants and chemorepellents. The MCD vastly accelerates the chemotactic screening process, which is critical to deciphering the complex sea of chemical stimuli underlying microbial responses. eLife Sciences Publications, Ltd 2023-07-24 /pmc/articles/PMC10365836/ /pubmed/37486823 http://dx.doi.org/10.7554/eLife.85348 Text en © 2023, Stehnach et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Ecology
Stehnach, Michael R
Henshaw, Richard J
Floge, Sheri A
Guasto, Jeffrey S
Multiplexed microfluidic screening of bacterial chemotaxis
title Multiplexed microfluidic screening of bacterial chemotaxis
title_full Multiplexed microfluidic screening of bacterial chemotaxis
title_fullStr Multiplexed microfluidic screening of bacterial chemotaxis
title_full_unstemmed Multiplexed microfluidic screening of bacterial chemotaxis
title_short Multiplexed microfluidic screening of bacterial chemotaxis
title_sort multiplexed microfluidic screening of bacterial chemotaxis
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365836/
https://www.ncbi.nlm.nih.gov/pubmed/37486823
http://dx.doi.org/10.7554/eLife.85348
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