Protocol for automated multivariate quantitative-image-based cytometry analysis by fluorescence microscopy of asynchronous adherent cells

Here, we present a protocol for multivariate quantitative-image-based cytometry (QIBC) analysis by fluorescence microscopy of asynchronous adherent cells. We describe steps for the preparation, treatment, and fixation of cells, sample staining, and imaging for QIBC. We then detail image analysis wit...

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Detalles Bibliográficos
Autores principales: Besse, Laetitia, Rumiac, Typhaine, Reynaud-Angelin, Anne, Messaoudi, Cédric, Soler, Marie-Noëlle, Lambert, Sarah A.E., Pennaneach, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365954/
https://www.ncbi.nlm.nih.gov/pubmed/37453067
http://dx.doi.org/10.1016/j.xpro.2023.102446

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