A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro

Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-l...

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Detalles Bibliográficos
Autores principales: Mao, Yong, John, Nikita, Protzman, Nicole M., Long, Desiree, Sivalenka, Raja, Azimi, Shamshad, Mirabile, Brandon, Pouliot, Robert, Gosiewska, Anna, Hariri, Robert J., Brigido, Stephen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Tissue Engineering Constructs and Cell Substrates
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366303/
https://www.ncbi.nlm.nih.gov/pubmed/37486403
http://dx.doi.org/10.1007/s10856-023-06740-4
Descripción
Sumario:Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity. GRAPHICAL ABSTRACT: [Image: see text]

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