A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro

Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-l...

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Autores principales: Mao, Yong, John, Nikita, Protzman, Nicole M., Long, Desiree, Sivalenka, Raja, Azimi, Shamshad, Mirabile, Brandon, Pouliot, Robert, Gosiewska, Anna, Hariri, Robert J., Brigido, Stephen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Tissue Engineering Constructs and Cell Substrates
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366303/
https://www.ncbi.nlm.nih.gov/pubmed/37486403
http://dx.doi.org/10.1007/s10856-023-06740-4
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author Mao, Yong
John, Nikita
Protzman, Nicole M.
Long, Desiree
Sivalenka, Raja
Azimi, Shamshad
Mirabile, Brandon
Pouliot, Robert
Gosiewska, Anna
Hariri, Robert J.
Brigido, Stephen A.
author_facet Mao, Yong
John, Nikita
Protzman, Nicole M.
Long, Desiree
Sivalenka, Raja
Azimi, Shamshad
Mirabile, Brandon
Pouliot, Robert
Gosiewska, Anna
Hariri, Robert J.
Brigido, Stephen A.
author_sort Mao, Yong
collection PubMed
description Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-103663032023-07-26 A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro Mao, Yong John, Nikita Protzman, Nicole M. Long, Desiree Sivalenka, Raja Azimi, Shamshad Mirabile, Brandon Pouliot, Robert Gosiewska, Anna Hariri, Robert J. Brigido, Stephen A. J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity. GRAPHICAL ABSTRACT: [Image: see text] Springer US 2023-07-24 2023 /pmc/articles/PMC10366303/ /pubmed/37486403 http://dx.doi.org/10.1007/s10856-023-06740-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Tissue Engineering Constructs and Cell Substrates
Mao, Yong
John, Nikita
Protzman, Nicole M.
Long, Desiree
Sivalenka, Raja
Azimi, Shamshad
Mirabile, Brandon
Pouliot, Robert
Gosiewska, Anna
Hariri, Robert J.
Brigido, Stephen A.
A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
title A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
title_full A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
title_fullStr A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
title_full_unstemmed A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
title_short A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
title_sort tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro
topic Tissue Engineering Constructs and Cell Substrates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366303/
https://www.ncbi.nlm.nih.gov/pubmed/37486403
http://dx.doi.org/10.1007/s10856-023-06740-4
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