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Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in living cells. Here, NanoLuc Binary Technology (NanoBiT) in...

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Detalles Bibliográficos
Autores principales:	Ogrodzinski, Lydia, Platt, Simon, Goulding, Joelle, Alexander, Cameron, Farr, Tracy D., Woolard, Jeanette, Hill, Stephen J., Kilpatrick, Laura E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366498/ https://www.ncbi.nlm.nih.gov/pubmed/37496673 http://dx.doi.org/10.1016/j.isci.2023.107232

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