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EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry
Epidermal Growth Factor Receptor (EGFR) overexpression or its mutation mediates the sustaining proliferative signaling, which is an important hallmark of cancer. Human EGFR-targeting monoclonal antibody (mAb) therapy such as cetuximab has been approved for clinical use in patients with colorectal ca...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366908/ https://www.ncbi.nlm.nih.gov/pubmed/37489364 http://dx.doi.org/10.3390/antib12030042 |
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author | Goto, Nohara Suzuki, Hiroyuki Tanaka, Tomohiro Ishikawa, Kenichiro Ouchida, Tsunenori Kaneko, Mika K. Kato, Yukinari |
author_facet | Goto, Nohara Suzuki, Hiroyuki Tanaka, Tomohiro Ishikawa, Kenichiro Ouchida, Tsunenori Kaneko, Mika K. Kato, Yukinari |
author_sort | Goto, Nohara |
collection | PubMed |
description | Epidermal Growth Factor Receptor (EGFR) overexpression or its mutation mediates the sustaining proliferative signaling, which is an important hallmark of cancer. Human EGFR-targeting monoclonal antibody (mAb) therapy such as cetuximab has been approved for clinical use in patients with colorectal cancers and head and neck squamous cell carcinomas. A reliable preclinical mouse model is essential to further develop the mAb therapy against EGFR. Therefore, sensitive mAbs against mouse EGFR (mEGFR) should be established. In this study, we developed a specific and sensitive mAb for mEGFR using the Cell-Based Immunization and Screening (CBIS) method. The established anti-mEGFR mAb, EMab-300 (rat IgG(1), kappa), reacted with mEGFR-overexpressed Chinese hamster ovary-K1 (CHO/mEGFR) and endogenously mEGFR-expressed cell lines, including NMuMG (a mouse mammary gland epithelial cell) and Lewis lung carcinoma cells, using flow cytometry. The kinetic analysis using flow cytometry indicated that the K(D) of EMab-300 for CHO/mEGFR and NMuMG was 4.3 × 10(−8) M and 1.9 × 10(−8) M, respectively. These results indicated that EMab-300 applies to the detection of mEGFR using flow cytometry and may be useful to obtain the proof of concept in preclinical studies. |
format | Online Article Text |
id | pubmed-10366908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103669082023-07-26 EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry Goto, Nohara Suzuki, Hiroyuki Tanaka, Tomohiro Ishikawa, Kenichiro Ouchida, Tsunenori Kaneko, Mika K. Kato, Yukinari Antibodies (Basel) Communication Epidermal Growth Factor Receptor (EGFR) overexpression or its mutation mediates the sustaining proliferative signaling, which is an important hallmark of cancer. Human EGFR-targeting monoclonal antibody (mAb) therapy such as cetuximab has been approved for clinical use in patients with colorectal cancers and head and neck squamous cell carcinomas. A reliable preclinical mouse model is essential to further develop the mAb therapy against EGFR. Therefore, sensitive mAbs against mouse EGFR (mEGFR) should be established. In this study, we developed a specific and sensitive mAb for mEGFR using the Cell-Based Immunization and Screening (CBIS) method. The established anti-mEGFR mAb, EMab-300 (rat IgG(1), kappa), reacted with mEGFR-overexpressed Chinese hamster ovary-K1 (CHO/mEGFR) and endogenously mEGFR-expressed cell lines, including NMuMG (a mouse mammary gland epithelial cell) and Lewis lung carcinoma cells, using flow cytometry. The kinetic analysis using flow cytometry indicated that the K(D) of EMab-300 for CHO/mEGFR and NMuMG was 4.3 × 10(−8) M and 1.9 × 10(−8) M, respectively. These results indicated that EMab-300 applies to the detection of mEGFR using flow cytometry and may be useful to obtain the proof of concept in preclinical studies. MDPI 2023-06-21 /pmc/articles/PMC10366908/ /pubmed/37489364 http://dx.doi.org/10.3390/antib12030042 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Goto, Nohara Suzuki, Hiroyuki Tanaka, Tomohiro Ishikawa, Kenichiro Ouchida, Tsunenori Kaneko, Mika K. Kato, Yukinari EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry |
title | EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry |
title_full | EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry |
title_fullStr | EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry |
title_full_unstemmed | EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry |
title_short | EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry |
title_sort | emab-300 detects mouse epidermal growth factor receptor-expressing cancer cell lines in flow cytometry |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366908/ https://www.ncbi.nlm.nih.gov/pubmed/37489364 http://dx.doi.org/10.3390/antib12030042 |
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