Cargando…

Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice

The crucial role of plasmid-encoded protein Pgp3 in Chlamydia pathogenesis has been demonstrated in various animal models. Previous studies have revealed that the Pgp3-deficient C. muridarum mutant fails to induce hydrosalpinx after vaginal inoculation in mice. Structural analysis of C. trachomatis...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Yumeng, Wu, Haoqing, Sun, Yina, Liu, Yuanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10367112/
https://www.ncbi.nlm.nih.gov/pubmed/37497542
http://dx.doi.org/10.3389/fmicb.2023.1216372
_version_ 1785077318200328192
author Huang, Yumeng
Wu, Haoqing
Sun, Yina
Liu, Yuanjun
author_facet Huang, Yumeng
Wu, Haoqing
Sun, Yina
Liu, Yuanjun
author_sort Huang, Yumeng
collection PubMed
description The crucial role of plasmid-encoded protein Pgp3 in Chlamydia pathogenesis has been demonstrated in various animal models. Previous studies have revealed that the Pgp3-deficient C. muridarum mutant fails to induce hydrosalpinx after vaginal inoculation in mice. Structural analysis of C. trachomatis Pgp3 trimer has indicated that Trp234 may play a critical role in trimeric crystal packing interactions and that Tyr197 is involved at predominant cation-binding sites. In this study, we constructed C. muridarum transformants harboring Pgp3, Trp234, or Tyr197 point mutations (Pgp3W234A and Pgp3Y197A). C3H/HeJ mice infected with Pgp3W234A mutant failed to induce severe hydrosalpinx in the oviduct tissue, which largely phenocopied the full-length Pgp3-deficient C. muridarum. The Pgp3Y197A variant induced an intermediate severity of pathology. The attenuated pathogenicity caused by the Pgp3W234A mutant may be due to its decreased survival in the lower genital tracts of mice, reduced ascension to the oviduct, and milder induction of inflammatory cell infiltration in the oviduct tissue. Thus, our results point to an important amino acid residue involved in Pgp3 virulence, providing a potential therapeutic target for chlamydial infection.
format Online
Article
Text
id pubmed-10367112
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-103671122023-07-26 Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice Huang, Yumeng Wu, Haoqing Sun, Yina Liu, Yuanjun Front Microbiol Microbiology The crucial role of plasmid-encoded protein Pgp3 in Chlamydia pathogenesis has been demonstrated in various animal models. Previous studies have revealed that the Pgp3-deficient C. muridarum mutant fails to induce hydrosalpinx after vaginal inoculation in mice. Structural analysis of C. trachomatis Pgp3 trimer has indicated that Trp234 may play a critical role in trimeric crystal packing interactions and that Tyr197 is involved at predominant cation-binding sites. In this study, we constructed C. muridarum transformants harboring Pgp3, Trp234, or Tyr197 point mutations (Pgp3W234A and Pgp3Y197A). C3H/HeJ mice infected with Pgp3W234A mutant failed to induce severe hydrosalpinx in the oviduct tissue, which largely phenocopied the full-length Pgp3-deficient C. muridarum. The Pgp3Y197A variant induced an intermediate severity of pathology. The attenuated pathogenicity caused by the Pgp3W234A mutant may be due to its decreased survival in the lower genital tracts of mice, reduced ascension to the oviduct, and milder induction of inflammatory cell infiltration in the oviduct tissue. Thus, our results point to an important amino acid residue involved in Pgp3 virulence, providing a potential therapeutic target for chlamydial infection. Frontiers Media S.A. 2023-07-11 /pmc/articles/PMC10367112/ /pubmed/37497542 http://dx.doi.org/10.3389/fmicb.2023.1216372 Text en Copyright © 2023 Huang, Wu, Sun and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Yumeng
Wu, Haoqing
Sun, Yina
Liu, Yuanjun
Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice
title Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice
title_full Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice
title_fullStr Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice
title_full_unstemmed Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice
title_short Tryptophan residue of plasmid-encoded Pgp3 is important for Chlamydia muridarum to induce hydrosalpinx in mice
title_sort tryptophan residue of plasmid-encoded pgp3 is important for chlamydia muridarum to induce hydrosalpinx in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10367112/
https://www.ncbi.nlm.nih.gov/pubmed/37497542
http://dx.doi.org/10.3389/fmicb.2023.1216372
work_keys_str_mv AT huangyumeng tryptophanresidueofplasmidencodedpgp3isimportantforchlamydiamuridarumtoinducehydrosalpinxinmice
AT wuhaoqing tryptophanresidueofplasmidencodedpgp3isimportantforchlamydiamuridarumtoinducehydrosalpinxinmice
AT sunyina tryptophanresidueofplasmidencodedpgp3isimportantforchlamydiamuridarumtoinducehydrosalpinxinmice
AT liuyuanjun tryptophanresidueofplasmidencodedpgp3isimportantforchlamydiamuridarumtoinducehydrosalpinxinmice