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Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation
Restoration of nerve tissue remains highly challenging, mainly due to the limited regeneration capacity of the nervous system and the development of fibrosis. This limitation necessitates designing new nerve guidance channel to promote nerve repairing. In this study, we developed a novel core/shell...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10368663/ https://www.ncbi.nlm.nih.gov/pubmed/37491480 http://dx.doi.org/10.1038/s41598-023-38456-4 |
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author | Javidi, Hamideh Ramazani Saadatabadi, Ahmad Sadrnezhaad, S. K. Najmoddin, Najmeh |
author_facet | Javidi, Hamideh Ramazani Saadatabadi, Ahmad Sadrnezhaad, S. K. Najmoddin, Najmeh |
author_sort | Javidi, Hamideh |
collection | PubMed |
description | Restoration of nerve tissue remains highly challenging, mainly due to the limited regeneration capacity of the nervous system and the development of fibrosis. This limitation necessitates designing new nerve guidance channel to promote nerve repairing. In this study, we developed a novel core/shell conduit to induce PC12 differentiation. Co-electrospinning method was utilized to produce a fibrous shell containing polycaprolactone/polyvinylidene fluoride PCL/PVDF, gelatin and polyaniline/graphene (PAG) nanocomposite. The core section of the conduit was filled with chitosan–gelatin hydrogel containing PAG and ZnO nanoparticles. Such conduit shows antibacterial activity, electrical conductivity and piezoelectric property. The effect of such engineered conduit on PC12 differentiation was investigated by analyzing differentiation markers Nestin and microtubule-associated protein 2 (MAP2) through immunocytochemistry and PCR-RT techniques. The result revealed that such conduit could significantly induce Nestin and MAP2 gene expression in the PC12 cells and, thus, it is a viable option for effective cell differentiation and nerve regeneration. |
format | Online Article Text |
id | pubmed-10368663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103686632023-07-27 Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation Javidi, Hamideh Ramazani Saadatabadi, Ahmad Sadrnezhaad, S. K. Najmoddin, Najmeh Sci Rep Article Restoration of nerve tissue remains highly challenging, mainly due to the limited regeneration capacity of the nervous system and the development of fibrosis. This limitation necessitates designing new nerve guidance channel to promote nerve repairing. In this study, we developed a novel core/shell conduit to induce PC12 differentiation. Co-electrospinning method was utilized to produce a fibrous shell containing polycaprolactone/polyvinylidene fluoride PCL/PVDF, gelatin and polyaniline/graphene (PAG) nanocomposite. The core section of the conduit was filled with chitosan–gelatin hydrogel containing PAG and ZnO nanoparticles. Such conduit shows antibacterial activity, electrical conductivity and piezoelectric property. The effect of such engineered conduit on PC12 differentiation was investigated by analyzing differentiation markers Nestin and microtubule-associated protein 2 (MAP2) through immunocytochemistry and PCR-RT techniques. The result revealed that such conduit could significantly induce Nestin and MAP2 gene expression in the PC12 cells and, thus, it is a viable option for effective cell differentiation and nerve regeneration. Nature Publishing Group UK 2023-07-25 /pmc/articles/PMC10368663/ /pubmed/37491480 http://dx.doi.org/10.1038/s41598-023-38456-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Javidi, Hamideh Ramazani Saadatabadi, Ahmad Sadrnezhaad, S. K. Najmoddin, Najmeh Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation |
title | Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation |
title_full | Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation |
title_fullStr | Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation |
title_full_unstemmed | Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation |
title_short | Conductive nerve conduit with piezoelectric properties for enhanced PC12 differentiation |
title_sort | conductive nerve conduit with piezoelectric properties for enhanced pc12 differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10368663/ https://www.ncbi.nlm.nih.gov/pubmed/37491480 http://dx.doi.org/10.1038/s41598-023-38456-4 |
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