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Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering

It is still a huge challenge for bone regenerative biomaterial to balance its mechanical, biological and biodegradable properties. In the present study, a new composite material including treated dentin matrix (TDM) and α-calcium sulphate hemihydrate (α-CSH) was prepared. The optimal composition rat...

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Autores principales: Guo, Runying, Zhang, Rui, Liu, Sirui, Yang, Yanyu, Dong, Wenhang, Wang, Meiyue, Mi, Hongyan, Liu, Mengzhe, Sun, Jingjing, Zhang, Xue, Su, Yimeng, Liu, Yiming, Huang, Di, Li, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369214/
https://www.ncbi.nlm.nih.gov/pubmed/37501676
http://dx.doi.org/10.1093/rb/rbad061
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author Guo, Runying
Zhang, Rui
Liu, Sirui
Yang, Yanyu
Dong, Wenhang
Wang, Meiyue
Mi, Hongyan
Liu, Mengzhe
Sun, Jingjing
Zhang, Xue
Su, Yimeng
Liu, Yiming
Huang, Di
Li, Rui
author_facet Guo, Runying
Zhang, Rui
Liu, Sirui
Yang, Yanyu
Dong, Wenhang
Wang, Meiyue
Mi, Hongyan
Liu, Mengzhe
Sun, Jingjing
Zhang, Xue
Su, Yimeng
Liu, Yiming
Huang, Di
Li, Rui
author_sort Guo, Runying
collection PubMed
description It is still a huge challenge for bone regenerative biomaterial to balance its mechanical, biological and biodegradable properties. In the present study, a new composite material including treated dentin matrix (TDM) and α-calcium sulphate hemihydrate (α-CSH) was prepared. The optimal composition ratio between TDM and α-CSH was explored. The results indicate that both components were physically mixed and structurally stable. Its compressive strength reaches up to 5.027 ± 0.035 MPa for 50%TDM/α-CSH group, similar to human cancellous bone tissues. Biological experiments results show that TDM/α-CSH composite exhibits excellent biocompatibility and the expression of osteogenic related genes and proteins (ALP, RUNX2, OPN) is significantly increased. In vivo experiments suggest that the addition of TDM for each group (10%, 30%, 50%) effectively promotes cell proliferation and osteomalacia. In addition, 50% of the TDM/α-CSH combination displays optimal osteoconductivity. The novel TDM/α-CSH composite is a good candidate for certain applications in bone tissue engineering.
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spelling pubmed-103692142023-07-27 Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering Guo, Runying Zhang, Rui Liu, Sirui Yang, Yanyu Dong, Wenhang Wang, Meiyue Mi, Hongyan Liu, Mengzhe Sun, Jingjing Zhang, Xue Su, Yimeng Liu, Yiming Huang, Di Li, Rui Regen Biomater Research Article It is still a huge challenge for bone regenerative biomaterial to balance its mechanical, biological and biodegradable properties. In the present study, a new composite material including treated dentin matrix (TDM) and α-calcium sulphate hemihydrate (α-CSH) was prepared. The optimal composition ratio between TDM and α-CSH was explored. The results indicate that both components were physically mixed and structurally stable. Its compressive strength reaches up to 5.027 ± 0.035 MPa for 50%TDM/α-CSH group, similar to human cancellous bone tissues. Biological experiments results show that TDM/α-CSH composite exhibits excellent biocompatibility and the expression of osteogenic related genes and proteins (ALP, RUNX2, OPN) is significantly increased. In vivo experiments suggest that the addition of TDM for each group (10%, 30%, 50%) effectively promotes cell proliferation and osteomalacia. In addition, 50% of the TDM/α-CSH combination displays optimal osteoconductivity. The novel TDM/α-CSH composite is a good candidate for certain applications in bone tissue engineering. Oxford University Press 2023-06-19 /pmc/articles/PMC10369214/ /pubmed/37501676 http://dx.doi.org/10.1093/rb/rbad061 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guo, Runying
Zhang, Rui
Liu, Sirui
Yang, Yanyu
Dong, Wenhang
Wang, Meiyue
Mi, Hongyan
Liu, Mengzhe
Sun, Jingjing
Zhang, Xue
Su, Yimeng
Liu, Yiming
Huang, Di
Li, Rui
Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
title Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
title_full Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
title_fullStr Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
title_full_unstemmed Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
title_short Biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
title_sort biomimetic, biodegradable and osteoinductive treated dentin matrix/α-calcium sulphate hemihydrate composite material for bone tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369214/
https://www.ncbi.nlm.nih.gov/pubmed/37501676
http://dx.doi.org/10.1093/rb/rbad061
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