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Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics
[Image: see text] Super-resolution imaging techniques have broken the diffraction-limited resolution of light microscopy. However, acquiring three-dimensional (3D) super-resolution information about structures and dynamic processes in live cells at high speed remains challenging. Recently, the devel...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nanjing University and American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369678/ https://www.ncbi.nlm.nih.gov/pubmed/37501792 http://dx.doi.org/10.1021/cbmi.3c00036 |
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author | Yu, Wenlan Rush, Coby Tingey, Mark Junod, Samuel Yang, Weidong |
author_facet | Yu, Wenlan Rush, Coby Tingey, Mark Junod, Samuel Yang, Weidong |
author_sort | Yu, Wenlan |
collection | PubMed |
description | [Image: see text] Super-resolution imaging techniques have broken the diffraction-limited resolution of light microscopy. However, acquiring three-dimensional (3D) super-resolution information about structures and dynamic processes in live cells at high speed remains challenging. Recently, the development of high-speed single-point edge-excitation subdiffraction (SPEED) microscopy, along with its 2D-to-3D transformation algorithm, provides a practical and effective approach to achieving 3D subdiffraction-limit information in subcellular structures and organelles with rotational symmetry. One of the major benefits of SPEED microscopy is that it does not rely on complex optical components and can be implemented on a standard, inverted epifluorescence microscope, simplifying the process of sample preparation and the expertise requirement. SPEED microscopy is specifically designed to obtain 2D spatial locations of individual immobile or moving fluorescent molecules inside submicrometer biological channels or cavities at high spatiotemporal resolution. The collected data are then subjected to postlocalization 2D-to-3D transformation to obtain 3D super-resolution structural and dynamic information. In recent years, SPEED microscopy has provided significant insights into nucleocytoplasmic transport across the nuclear pore complex (NPC) and cytoplasm-cilium trafficking through the ciliary transition zone. This Review focuses on the applications of SPEED microscopy in studying the structure and function of nuclear pores. |
format | Online Article Text |
id | pubmed-10369678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nanjing University and American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-103696782023-07-27 Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics Yu, Wenlan Rush, Coby Tingey, Mark Junod, Samuel Yang, Weidong Chem Biomed Imaging [Image: see text] Super-resolution imaging techniques have broken the diffraction-limited resolution of light microscopy. However, acquiring three-dimensional (3D) super-resolution information about structures and dynamic processes in live cells at high speed remains challenging. Recently, the development of high-speed single-point edge-excitation subdiffraction (SPEED) microscopy, along with its 2D-to-3D transformation algorithm, provides a practical and effective approach to achieving 3D subdiffraction-limit information in subcellular structures and organelles with rotational symmetry. One of the major benefits of SPEED microscopy is that it does not rely on complex optical components and can be implemented on a standard, inverted epifluorescence microscope, simplifying the process of sample preparation and the expertise requirement. SPEED microscopy is specifically designed to obtain 2D spatial locations of individual immobile or moving fluorescent molecules inside submicrometer biological channels or cavities at high spatiotemporal resolution. The collected data are then subjected to postlocalization 2D-to-3D transformation to obtain 3D super-resolution structural and dynamic information. In recent years, SPEED microscopy has provided significant insights into nucleocytoplasmic transport across the nuclear pore complex (NPC) and cytoplasm-cilium trafficking through the ciliary transition zone. This Review focuses on the applications of SPEED microscopy in studying the structure and function of nuclear pores. Nanjing University and American Chemical Society 2023-06-24 /pmc/articles/PMC10369678/ /pubmed/37501792 http://dx.doi.org/10.1021/cbmi.3c00036 Text en © 2023 The Authors. Co-published by Nanjing University and American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Yu, Wenlan Rush, Coby Tingey, Mark Junod, Samuel Yang, Weidong Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics |
title | Application of Super-resolution SPEED Microscopy in
the Study of Cellular Dynamics |
title_full | Application of Super-resolution SPEED Microscopy in
the Study of Cellular Dynamics |
title_fullStr | Application of Super-resolution SPEED Microscopy in
the Study of Cellular Dynamics |
title_full_unstemmed | Application of Super-resolution SPEED Microscopy in
the Study of Cellular Dynamics |
title_short | Application of Super-resolution SPEED Microscopy in
the Study of Cellular Dynamics |
title_sort | application of super-resolution speed microscopy in
the study of cellular dynamics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369678/ https://www.ncbi.nlm.nih.gov/pubmed/37501792 http://dx.doi.org/10.1021/cbmi.3c00036 |
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