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The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts

Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multiprotein complexes. The RNA-binding protein ZC3H11A is involved in nuclear mRNA export, NF-κB signaling, and is essential during mouse embryo development. Furthermore, previous studies have shown...

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Autores principales: Kases, Katharina, Schubert, Erik, Hajikhezri, Zamaneh, Larsson, Mårten, Devi, Priya, Darweesh, Mahmoud, Andersson, Leif, Akusjärvi, Göran, Punga, Tanel, Younis, Shady
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371797/
https://www.ncbi.nlm.nih.gov/pubmed/37356722
http://dx.doi.org/10.1016/j.jbc.2023.104959
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author Kases, Katharina
Schubert, Erik
Hajikhezri, Zamaneh
Larsson, Mårten
Devi, Priya
Darweesh, Mahmoud
Andersson, Leif
Akusjärvi, Göran
Punga, Tanel
Younis, Shady
author_facet Kases, Katharina
Schubert, Erik
Hajikhezri, Zamaneh
Larsson, Mårten
Devi, Priya
Darweesh, Mahmoud
Andersson, Leif
Akusjärvi, Göran
Punga, Tanel
Younis, Shady
author_sort Kases, Katharina
collection PubMed
description Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multiprotein complexes. The RNA-binding protein ZC3H11A is involved in nuclear mRNA export, NF-κB signaling, and is essential during mouse embryo development. Furthermore, previous studies have shown that ZC3H11A is important for nuclear-replicating viruses. However, detailed biochemical characterization of the ZC3H11A protein has been lacking. In this study, we established the ZC3H11A protein interactome in human and mouse cells. We demonstrate that the nuclear poly(A)-binding protein PABPN1 interacts specifically with the ZC3H11A protein and controls ZC3H11A localization into nuclear speckles. We report that ZC3H11A specifically interacts with the human adenovirus type 5 (HAdV-5) capsid mRNA in a PABPN1-dependent manner. Notably, ZC3H11A uses the same zinc finger motifs to interact with PABPN1 and viral mRNA. Further, we demonstrate that the lack of ZC3H11A alters the polyadenylation of HAdV-5 capsid mRNA. Taken together, our results suggest that the ZC3H11A protein may act as a novel regulator of polyadenylation of nuclear mRNA.
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spelling pubmed-103717972023-07-28 The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts Kases, Katharina Schubert, Erik Hajikhezri, Zamaneh Larsson, Mårten Devi, Priya Darweesh, Mahmoud Andersson, Leif Akusjärvi, Göran Punga, Tanel Younis, Shady J Biol Chem Research Article Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multiprotein complexes. The RNA-binding protein ZC3H11A is involved in nuclear mRNA export, NF-κB signaling, and is essential during mouse embryo development. Furthermore, previous studies have shown that ZC3H11A is important for nuclear-replicating viruses. However, detailed biochemical characterization of the ZC3H11A protein has been lacking. In this study, we established the ZC3H11A protein interactome in human and mouse cells. We demonstrate that the nuclear poly(A)-binding protein PABPN1 interacts specifically with the ZC3H11A protein and controls ZC3H11A localization into nuclear speckles. We report that ZC3H11A specifically interacts with the human adenovirus type 5 (HAdV-5) capsid mRNA in a PABPN1-dependent manner. Notably, ZC3H11A uses the same zinc finger motifs to interact with PABPN1 and viral mRNA. Further, we demonstrate that the lack of ZC3H11A alters the polyadenylation of HAdV-5 capsid mRNA. Taken together, our results suggest that the ZC3H11A protein may act as a novel regulator of polyadenylation of nuclear mRNA. American Society for Biochemistry and Molecular Biology 2023-06-23 /pmc/articles/PMC10371797/ /pubmed/37356722 http://dx.doi.org/10.1016/j.jbc.2023.104959 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Kases, Katharina
Schubert, Erik
Hajikhezri, Zamaneh
Larsson, Mårten
Devi, Priya
Darweesh, Mahmoud
Andersson, Leif
Akusjärvi, Göran
Punga, Tanel
Younis, Shady
The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
title The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
title_full The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
title_fullStr The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
title_full_unstemmed The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
title_short The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
title_sort rna-binding protein zc3h11a interacts with the nuclear poly(a)-binding protein pabpn1 and alters polyadenylation of viral transcripts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371797/
https://www.ncbi.nlm.nih.gov/pubmed/37356722
http://dx.doi.org/10.1016/j.jbc.2023.104959
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