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Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP

Chronic rhinosinusitis with nasal polyps (CRSwNP) is defined as a Type 2 eosinophilic disease, while CRSsNP is considered a Type 1 neutrophilic disease. Since neutrophils are also activated in eosinophilic CRSwNP, the eosinophil–neutrophil dualism has been revaluated. Among the inflammatory cells in...

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Autores principales: Gelardi, Matteo, Giancaspro, Rossana, Duda, Loren, Quaranta, Vitaliano Nicola, Pizzulli, Cristina, Maiorano, Eugenio, Di Canio, Filomena Milena, Ruzza, Annamaria, Iannuzzi, Lucia, Quaranta, Nicola Antonio Adolfo, Parisi, Francesca, Cassano, Michele, Marzullo, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372103/
https://www.ncbi.nlm.nih.gov/pubmed/37495667
http://dx.doi.org/10.1038/s41598-023-39149-8
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author Gelardi, Matteo
Giancaspro, Rossana
Duda, Loren
Quaranta, Vitaliano Nicola
Pizzulli, Cristina
Maiorano, Eugenio
Di Canio, Filomena Milena
Ruzza, Annamaria
Iannuzzi, Lucia
Quaranta, Nicola Antonio Adolfo
Parisi, Francesca
Cassano, Michele
Marzullo, Andrea
author_facet Gelardi, Matteo
Giancaspro, Rossana
Duda, Loren
Quaranta, Vitaliano Nicola
Pizzulli, Cristina
Maiorano, Eugenio
Di Canio, Filomena Milena
Ruzza, Annamaria
Iannuzzi, Lucia
Quaranta, Nicola Antonio Adolfo
Parisi, Francesca
Cassano, Michele
Marzullo, Andrea
author_sort Gelardi, Matteo
collection PubMed
description Chronic rhinosinusitis with nasal polyps (CRSwNP) is defined as a Type 2 eosinophilic disease, while CRSsNP is considered a Type 1 neutrophilic disease. Since neutrophils are also activated in eosinophilic CRSwNP, the eosinophil–neutrophil dualism has been revaluated. Among the inflammatory cells infiltrating sinus-nasal tissues, the role of mast cells (MCs) is not already recognized, although Clinical-Cytological Grading, which defines the severity of CRSwNP, attributes to mixed eosinophil-MC forms of CRSwNP a greater risk of recurrence. We aimed to examine nasal polyps from both a cytological and histopathological point of view, to evaluate the presence and localization of MCs. Cytological and histological examination of 39 samples of nasal polyps were performed. Immunohistochemistry was used to evaluate the presence of Tryptase + CD117 + MCs, which were counted both in the epithelial layer and in the lamina propria. A statistically significant correlation was found between intraepithelial MCs and CRSwNP severity (p < 0.001) and between the total eosinophil count and the total mast cell count (p < 0.001). Cytological examination and immunohistochemistry were comparable in detecting the presence of intraepithelial MCs (p = 0.002). The histological cut-off of 6 intraepithelial MCs was identified to detect severe CRSwNP (p < 0.001). MCs have been shown to be located in the lamina propria of almost all eosinophilic nasal polyps without significantly affecting their severity. Intraepithelial MCs are associated with greater severity of CRSwNP. Histopathological criteria of the eosinophil-MC form of CRSwNP in addition to the eosinophilic one, should be defined to guarantee patients effective and tailored treatments.
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spelling pubmed-103721032023-07-28 Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP Gelardi, Matteo Giancaspro, Rossana Duda, Loren Quaranta, Vitaliano Nicola Pizzulli, Cristina Maiorano, Eugenio Di Canio, Filomena Milena Ruzza, Annamaria Iannuzzi, Lucia Quaranta, Nicola Antonio Adolfo Parisi, Francesca Cassano, Michele Marzullo, Andrea Sci Rep Article Chronic rhinosinusitis with nasal polyps (CRSwNP) is defined as a Type 2 eosinophilic disease, while CRSsNP is considered a Type 1 neutrophilic disease. Since neutrophils are also activated in eosinophilic CRSwNP, the eosinophil–neutrophil dualism has been revaluated. Among the inflammatory cells infiltrating sinus-nasal tissues, the role of mast cells (MCs) is not already recognized, although Clinical-Cytological Grading, which defines the severity of CRSwNP, attributes to mixed eosinophil-MC forms of CRSwNP a greater risk of recurrence. We aimed to examine nasal polyps from both a cytological and histopathological point of view, to evaluate the presence and localization of MCs. Cytological and histological examination of 39 samples of nasal polyps were performed. Immunohistochemistry was used to evaluate the presence of Tryptase + CD117 + MCs, which were counted both in the epithelial layer and in the lamina propria. A statistically significant correlation was found between intraepithelial MCs and CRSwNP severity (p < 0.001) and between the total eosinophil count and the total mast cell count (p < 0.001). Cytological examination and immunohistochemistry were comparable in detecting the presence of intraepithelial MCs (p = 0.002). The histological cut-off of 6 intraepithelial MCs was identified to detect severe CRSwNP (p < 0.001). MCs have been shown to be located in the lamina propria of almost all eosinophilic nasal polyps without significantly affecting their severity. Intraepithelial MCs are associated with greater severity of CRSwNP. Histopathological criteria of the eosinophil-MC form of CRSwNP in addition to the eosinophilic one, should be defined to guarantee patients effective and tailored treatments. Nature Publishing Group UK 2023-07-26 /pmc/articles/PMC10372103/ /pubmed/37495667 http://dx.doi.org/10.1038/s41598-023-39149-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gelardi, Matteo
Giancaspro, Rossana
Duda, Loren
Quaranta, Vitaliano Nicola
Pizzulli, Cristina
Maiorano, Eugenio
Di Canio, Filomena Milena
Ruzza, Annamaria
Iannuzzi, Lucia
Quaranta, Nicola Antonio Adolfo
Parisi, Francesca
Cassano, Michele
Marzullo, Andrea
Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP
title Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP
title_full Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP
title_fullStr Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP
title_full_unstemmed Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP
title_short Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP
title_sort eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in crswnp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372103/
https://www.ncbi.nlm.nih.gov/pubmed/37495667
http://dx.doi.org/10.1038/s41598-023-39149-8
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