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A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
The lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries....
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372488/ https://www.ncbi.nlm.nih.gov/pubmed/37521935 http://dx.doi.org/10.3389/fpls.2023.1212979 |
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author | Simiyu, David Charles Jang, Jin Hoon Lee, Ok Ran |
author_facet | Simiyu, David Charles Jang, Jin Hoon Lee, Ok Ran |
author_sort | Simiyu, David Charles |
collection | PubMed |
description | The lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries. Thus, plants with less lignin are ideal for usage in such industries. Molecular studies have identified genes that regulate plant lignification, including group III plant-specific patatin-related phospholipase genes. Recent studies have reported decreased lignin content when pPLAIIIα, pPLAIIIγ (from Arabidopsis thaliana), and pPLAIIIβ (from Panax ginseng) were overexpressed in Arabidopsis. However, the role played by a closely related gene pPLAIIIδ in lignin biosynthesis has not yet been reported. In this study, we found that overexpression of the pPLAIIIδ significantly reduced the lignin content in secondary cell walls, whereas the silencing of the gene increased secondary walls lignification. Transcript level analysis showed that the key structural and regulatory genes involved in the lignin biosynthesis pathway decreased in overexpression, and increased in plants with silenced pPLAIIIδ. Further analysis revealed that pPLAIIIδ played an influential role in several physiological processes including seed germination, and chlorophyll accumulation. Moreover, the gene also influenced the size of plants and plant organs, including leaves, seeds, and root hairs. Generally, our study provides important insights toward the use of genetic engineering for lignin reduction in plants and provides information about the agronomical and physiological suitability of pPLAIIIδ transgenic plants for utilization in biomass processing industries. |
format | Online Article Text |
id | pubmed-10372488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103724882023-07-28 A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana Simiyu, David Charles Jang, Jin Hoon Lee, Ok Ran Front Plant Sci Plant Science The lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries. Thus, plants with less lignin are ideal for usage in such industries. Molecular studies have identified genes that regulate plant lignification, including group III plant-specific patatin-related phospholipase genes. Recent studies have reported decreased lignin content when pPLAIIIα, pPLAIIIγ (from Arabidopsis thaliana), and pPLAIIIβ (from Panax ginseng) were overexpressed in Arabidopsis. However, the role played by a closely related gene pPLAIIIδ in lignin biosynthesis has not yet been reported. In this study, we found that overexpression of the pPLAIIIδ significantly reduced the lignin content in secondary cell walls, whereas the silencing of the gene increased secondary walls lignification. Transcript level analysis showed that the key structural and regulatory genes involved in the lignin biosynthesis pathway decreased in overexpression, and increased in plants with silenced pPLAIIIδ. Further analysis revealed that pPLAIIIδ played an influential role in several physiological processes including seed germination, and chlorophyll accumulation. Moreover, the gene also influenced the size of plants and plant organs, including leaves, seeds, and root hairs. Generally, our study provides important insights toward the use of genetic engineering for lignin reduction in plants and provides information about the agronomical and physiological suitability of pPLAIIIδ transgenic plants for utilization in biomass processing industries. Frontiers Media S.A. 2023-07-13 /pmc/articles/PMC10372488/ /pubmed/37521935 http://dx.doi.org/10.3389/fpls.2023.1212979 Text en Copyright © 2023 Simiyu, Jang and Lee https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Simiyu, David Charles Jang, Jin Hoon Lee, Ok Ran A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana |
title | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
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title_full | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
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title_fullStr | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
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title_full_unstemmed | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
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title_short | A group III patatin-like phospholipase gene pPLAIIIδ regulates lignin biosynthesis and influences the rate of seed germination in Arabidopsis thaliana
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title_sort | group iii patatin-like phospholipase gene pplaiiiδ regulates lignin biosynthesis and influences the rate of seed germination in arabidopsis thaliana |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372488/ https://www.ncbi.nlm.nih.gov/pubmed/37521935 http://dx.doi.org/10.3389/fpls.2023.1212979 |
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