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Migration and division in cell monolayers on substrates with topological defects
Collective movement and organization of cell monolayers are important for wound healing and tissue development. Recent experiments highlighted the importance of liquid crystal order within these layers, suggesting that +1 topological defects have a role in organizing tissue morphogenesis. We study f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372565/ https://www.ncbi.nlm.nih.gov/pubmed/37463218 http://dx.doi.org/10.1073/pnas.2301197120 |
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author | Kaiyrbekov, Kurmanbek Endresen, Kirsten Sullivan, Kyle Zheng, Zhaofei Chen, Yun Serra, Francesca Camley, Brian A. |
author_facet | Kaiyrbekov, Kurmanbek Endresen, Kirsten Sullivan, Kyle Zheng, Zhaofei Chen, Yun Serra, Francesca Camley, Brian A. |
author_sort | Kaiyrbekov, Kurmanbek |
collection | PubMed |
description | Collective movement and organization of cell monolayers are important for wound healing and tissue development. Recent experiments highlighted the importance of liquid crystal order within these layers, suggesting that +1 topological defects have a role in organizing tissue morphogenesis. We study fibroblast organization, motion, and proliferation on a substrate with micron-sized ridges that induce +1 and −1 topological defects using simulation and experiment. We model cells as self-propelled deformable ellipses that interact via a Gay–Berne potential. Unlike earlier work on other cell types, we see that density variation near defects is not explained by collective migration. We propose instead that fibroblasts have different division rates depending on their area and aspect ratio. This model captures key features of our previous experiments: the alignment quality worsens at high cell density and, at the center of the +1 defects, cells can adopt either highly anisotropic or primarily isotropic morphologies. Experiments performed with different ridge heights confirm a prediction of this model: Suppressing migration across ridges promotes higher cell density at the +1 defect. Our work enables a mechanism for tissue patterning using topological defects without relying on cell migration. |
format | Online Article Text |
id | pubmed-10372565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-103725652023-07-28 Migration and division in cell monolayers on substrates with topological defects Kaiyrbekov, Kurmanbek Endresen, Kirsten Sullivan, Kyle Zheng, Zhaofei Chen, Yun Serra, Francesca Camley, Brian A. Proc Natl Acad Sci U S A Physical Sciences Collective movement and organization of cell monolayers are important for wound healing and tissue development. Recent experiments highlighted the importance of liquid crystal order within these layers, suggesting that +1 topological defects have a role in organizing tissue morphogenesis. We study fibroblast organization, motion, and proliferation on a substrate with micron-sized ridges that induce +1 and −1 topological defects using simulation and experiment. We model cells as self-propelled deformable ellipses that interact via a Gay–Berne potential. Unlike earlier work on other cell types, we see that density variation near defects is not explained by collective migration. We propose instead that fibroblasts have different division rates depending on their area and aspect ratio. This model captures key features of our previous experiments: the alignment quality worsens at high cell density and, at the center of the +1 defects, cells can adopt either highly anisotropic or primarily isotropic morphologies. Experiments performed with different ridge heights confirm a prediction of this model: Suppressing migration across ridges promotes higher cell density at the +1 defect. Our work enables a mechanism for tissue patterning using topological defects without relying on cell migration. National Academy of Sciences 2023-07-18 2023-07-25 /pmc/articles/PMC10372565/ /pubmed/37463218 http://dx.doi.org/10.1073/pnas.2301197120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Physical Sciences Kaiyrbekov, Kurmanbek Endresen, Kirsten Sullivan, Kyle Zheng, Zhaofei Chen, Yun Serra, Francesca Camley, Brian A. Migration and division in cell monolayers on substrates with topological defects |
title | Migration and division in cell monolayers on substrates with topological defects |
title_full | Migration and division in cell monolayers on substrates with topological defects |
title_fullStr | Migration and division in cell monolayers on substrates with topological defects |
title_full_unstemmed | Migration and division in cell monolayers on substrates with topological defects |
title_short | Migration and division in cell monolayers on substrates with topological defects |
title_sort | migration and division in cell monolayers on substrates with topological defects |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372565/ https://www.ncbi.nlm.nih.gov/pubmed/37463218 http://dx.doi.org/10.1073/pnas.2301197120 |
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