Cloning, purification and characterization of human dentine matrix protein 1(DMP1)

INTRODUCTION: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human...

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Detalles Bibliográficos
Autores principales: El-Bialy, Tarek, Aizenbud, Dror, Sadeghi, Hamid, Scott, Paul, Aizenbud, Itay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373089/
https://www.ncbi.nlm.nih.gov/pubmed/37520603
http://dx.doi.org/10.1016/j.sdentj.2023.05.006
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author El-Bialy, Tarek
Aizenbud, Dror
Sadeghi, Hamid
Scott, Paul
Aizenbud, Itay
author_facet El-Bialy, Tarek
Aizenbud, Dror
Sadeghi, Hamid
Scott, Paul
Aizenbud, Itay
author_sort El-Bialy, Tarek
collection PubMed
description INTRODUCTION: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. MATERIALS AND METHODS: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. RESULTS: The recombinant DMP1 was purified and characterized. CONCLUSION: The results suggested its future use in dental tissue regeneration and tissue engineering.
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spelling pubmed-103730892023-07-28 Cloning, purification and characterization of human dentine matrix protein 1(DMP1) El-Bialy, Tarek Aizenbud, Dror Sadeghi, Hamid Scott, Paul Aizenbud, Itay Saudi Dent J Original Article INTRODUCTION: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. MATERIALS AND METHODS: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. RESULTS: The recombinant DMP1 was purified and characterized. CONCLUSION: The results suggested its future use in dental tissue regeneration and tissue engineering. Elsevier 2023-07 2023-05-19 /pmc/articles/PMC10373089/ /pubmed/37520603 http://dx.doi.org/10.1016/j.sdentj.2023.05.006 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
El-Bialy, Tarek
Aizenbud, Dror
Sadeghi, Hamid
Scott, Paul
Aizenbud, Itay
Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
title Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
title_full Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
title_fullStr Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
title_full_unstemmed Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
title_short Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
title_sort cloning, purification and characterization of human dentine matrix protein 1(dmp1)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373089/
https://www.ncbi.nlm.nih.gov/pubmed/37520603
http://dx.doi.org/10.1016/j.sdentj.2023.05.006
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