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Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation
[Image: see text] Acetate is an end-product of anaerobic biodegradation and one of the major metabolites of microbial fermentation and lingo-cellulosic hydrolysate. Recently, acetate has been highlighted as a feedstock to produce value-added chemicals. This study examined acetate conversion to succi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373214/ https://www.ncbi.nlm.nih.gov/pubmed/37521642 http://dx.doi.org/10.1021/acsomega.3c02520 |
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author | Mutyala, Sakuntala Li, Shuwei Khandelwal, Himanshu Kong, Da Seul Kim, Jung Rae |
author_facet | Mutyala, Sakuntala Li, Shuwei Khandelwal, Himanshu Kong, Da Seul Kim, Jung Rae |
author_sort | Mutyala, Sakuntala |
collection | PubMed |
description | [Image: see text] Acetate is an end-product of anaerobic biodegradation and one of the major metabolites of microbial fermentation and lingo-cellulosic hydrolysate. Recently, acetate has been highlighted as a feedstock to produce value-added chemicals. This study examined acetate conversion to succinate by citrate synthase (gltA)-overexpressed Pseudomonas putida under microaerobic conditions. The acetate metabolism is initiated with the gltA enzyme, which converts acetyl-CoA to citrate. gltA-overexpressing P. putida (gltA-KT) showed an ∼50% improvement in succinate production compared to the wild type. Under the optimal pH of 7.5, the accumulation of succinate (4.73 ± 0.6 mM in 36 h) was ∼400% higher than that of the wild type. Overall, gltA overexpression alone resulted in 9.5% of the maximum theoretical yield in a minimal medium with acetate as the sole carbon source. This result shows that citrate synthase is important in acetate conversion to succinate by P. putida under microaerobic conditions. |
format | Online Article Text |
id | pubmed-10373214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-103732142023-07-28 Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation Mutyala, Sakuntala Li, Shuwei Khandelwal, Himanshu Kong, Da Seul Kim, Jung Rae ACS Omega [Image: see text] Acetate is an end-product of anaerobic biodegradation and one of the major metabolites of microbial fermentation and lingo-cellulosic hydrolysate. Recently, acetate has been highlighted as a feedstock to produce value-added chemicals. This study examined acetate conversion to succinate by citrate synthase (gltA)-overexpressed Pseudomonas putida under microaerobic conditions. The acetate metabolism is initiated with the gltA enzyme, which converts acetyl-CoA to citrate. gltA-overexpressing P. putida (gltA-KT) showed an ∼50% improvement in succinate production compared to the wild type. Under the optimal pH of 7.5, the accumulation of succinate (4.73 ± 0.6 mM in 36 h) was ∼400% higher than that of the wild type. Overall, gltA overexpression alone resulted in 9.5% of the maximum theoretical yield in a minimal medium with acetate as the sole carbon source. This result shows that citrate synthase is important in acetate conversion to succinate by P. putida under microaerobic conditions. American Chemical Society 2023-07-17 /pmc/articles/PMC10373214/ /pubmed/37521642 http://dx.doi.org/10.1021/acsomega.3c02520 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Mutyala, Sakuntala Li, Shuwei Khandelwal, Himanshu Kong, Da Seul Kim, Jung Rae Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation |
title | Citrate Synthase
Overexpression of Pseudomonas putida Increases Succinate Production
from Acetate in Microaerobic Cultivation |
title_full | Citrate Synthase
Overexpression of Pseudomonas putida Increases Succinate Production
from Acetate in Microaerobic Cultivation |
title_fullStr | Citrate Synthase
Overexpression of Pseudomonas putida Increases Succinate Production
from Acetate in Microaerobic Cultivation |
title_full_unstemmed | Citrate Synthase
Overexpression of Pseudomonas putida Increases Succinate Production
from Acetate in Microaerobic Cultivation |
title_short | Citrate Synthase
Overexpression of Pseudomonas putida Increases Succinate Production
from Acetate in Microaerobic Cultivation |
title_sort | citrate synthase
overexpression of pseudomonas putida increases succinate production
from acetate in microaerobic cultivation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373214/ https://www.ncbi.nlm.nih.gov/pubmed/37521642 http://dx.doi.org/10.1021/acsomega.3c02520 |
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