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Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study

AIM: To evaluate the remineralizing potential of natural substances on artificially induced caries lesions in primary teeth. MATERIALS AND METHODS: A total of 50 primary molar teeth were selected and subjected to a demineralization process. Then samples were randomly divided into five groups for the...

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Autores principales: Ravi, Pooja V, Sekar, Rajakumar, Ramar, Kavitha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Jaypee Brothers Medical Publishers 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373788/
https://www.ncbi.nlm.nih.gov/pubmed/37519954
http://dx.doi.org/10.5005/jp-journals-10005-2569
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author Ravi, Pooja V
Sekar, Rajakumar
Ramar, Kavitha
author_facet Ravi, Pooja V
Sekar, Rajakumar
Ramar, Kavitha
author_sort Ravi, Pooja V
collection PubMed
description AIM: To evaluate the remineralizing potential of natural substances on artificially induced caries lesions in primary teeth. MATERIALS AND METHODS: A total of 50 primary molar teeth were selected and subjected to a demineralization process. Then samples were randomly divided into five groups for the remineralization process. Group I—colophony, group II—5% sodium fluoride (NaF) + colophony, group III—grape seed extract (GSE) + colophony, group IV—5% NaF + colophony + 10% peptide, and group V— GSE + colophony + 10% peptide. All the groups were subjected to remineralization using a brushing stimulator for 3,000 cycles. Assessment was done using Vickers hardness testing machine for evaluating the enamel surface microhardness (SMH) and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX) for evaluating the surface morphology and mineral content, before and after demineralization and after remineralization, the obtained data was analyzed statistically using IBM Statistical Package for the Social Sciences (SPSS) software version 25. RESULTS: The enamel microhardness results of this study revealed that remineralization of enamel was highest in group V (212.83 ± 64.416) and least in group II (137.83 ± 26.324) p-value of 0.038. SEM-EDX analysis revealed high calcium (Ca) and fluoride (F) content in groups II and IV, which was significant (p-value of 0.001) from other groups. Surface morphology evaluated with SEM revealed spherical globular agglomerates and scaffolding deposits on the enamel surface in groups III and V resembling the remineralization process. CONCLUSION: Grape seed extract (GSE) with colophony and peptide is a superior natural alternative to NaF. Colophony also exhibited remineralizing potential in primary enamel. CLINICAL SIGNIFICANCE: Natural remineralizing agents like GSE, colophony, and its combination serves as a potential alternative to overcome the toxic effect on long-term usage of F. These natural substances can be applicable in clinical conditions by incorporating toothpaste and varnish, which can be used as an alternative or adjuvant to the topical application of F. HOW TO CITE THIS ARTICLE: Pooja V Ravi, Rajakumar S, Kavitha Ramar. Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study. Int J Clin Pediatr Dent 2023;16(2):244–250.
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spelling pubmed-103737882023-07-28 Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study Ravi, Pooja V Sekar, Rajakumar Ramar, Kavitha Int J Clin Pediatr Dent Original Research AIM: To evaluate the remineralizing potential of natural substances on artificially induced caries lesions in primary teeth. MATERIALS AND METHODS: A total of 50 primary molar teeth were selected and subjected to a demineralization process. Then samples were randomly divided into five groups for the remineralization process. Group I—colophony, group II—5% sodium fluoride (NaF) + colophony, group III—grape seed extract (GSE) + colophony, group IV—5% NaF + colophony + 10% peptide, and group V— GSE + colophony + 10% peptide. All the groups were subjected to remineralization using a brushing stimulator for 3,000 cycles. Assessment was done using Vickers hardness testing machine for evaluating the enamel surface microhardness (SMH) and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX) for evaluating the surface morphology and mineral content, before and after demineralization and after remineralization, the obtained data was analyzed statistically using IBM Statistical Package for the Social Sciences (SPSS) software version 25. RESULTS: The enamel microhardness results of this study revealed that remineralization of enamel was highest in group V (212.83 ± 64.416) and least in group II (137.83 ± 26.324) p-value of 0.038. SEM-EDX analysis revealed high calcium (Ca) and fluoride (F) content in groups II and IV, which was significant (p-value of 0.001) from other groups. Surface morphology evaluated with SEM revealed spherical globular agglomerates and scaffolding deposits on the enamel surface in groups III and V resembling the remineralization process. CONCLUSION: Grape seed extract (GSE) with colophony and peptide is a superior natural alternative to NaF. Colophony also exhibited remineralizing potential in primary enamel. CLINICAL SIGNIFICANCE: Natural remineralizing agents like GSE, colophony, and its combination serves as a potential alternative to overcome the toxic effect on long-term usage of F. These natural substances can be applicable in clinical conditions by incorporating toothpaste and varnish, which can be used as an alternative or adjuvant to the topical application of F. HOW TO CITE THIS ARTICLE: Pooja V Ravi, Rajakumar S, Kavitha Ramar. Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study. Int J Clin Pediatr Dent 2023;16(2):244–250. Jaypee Brothers Medical Publishers 2023 /pmc/articles/PMC10373788/ /pubmed/37519954 http://dx.doi.org/10.5005/jp-journals-10005-2569 Text en Copyright © 2023; The Author(s). https://creativecommons.org/licenses/by-nc/4.0/© The Author(s). 2023 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and non-commercial reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Original Research
Ravi, Pooja V
Sekar, Rajakumar
Ramar, Kavitha
Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study
title Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study
title_full Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study
title_fullStr Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study
title_full_unstemmed Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study
title_short Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study
title_sort evaluation of remineralization potential of natural substances on artificially induced carious lesions in primary teeth: an in vitro study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373788/
https://www.ncbi.nlm.nih.gov/pubmed/37519954
http://dx.doi.org/10.5005/jp-journals-10005-2569
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