Cargando…

Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting

Background: Cuproptosis is a novel cell death mechanism, and FDX1 is a key gene associated with cuproptosis. However, it is unclear whether FDX1 has prognostic and immunotherapeutic value for clear cell renal carcinoma (ccRCC). Methods: Data on FDX1 expression in ccRCC were extracted from various da...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Congbo, Zhou, Kena, Jing, Jing, Ren, Yu, Weng, Guobin, Cen, Dong, Wang, Xue, Huang, Shuaishuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373983/
https://www.ncbi.nlm.nih.gov/pubmed/37432054
http://dx.doi.org/10.18632/aging.204807
_version_ 1785078676064305152
author Cai, Congbo
Zhou, Kena
Jing, Jing
Ren, Yu
Weng, Guobin
Cen, Dong
Wang, Xue
Huang, Shuaishuai
author_facet Cai, Congbo
Zhou, Kena
Jing, Jing
Ren, Yu
Weng, Guobin
Cen, Dong
Wang, Xue
Huang, Shuaishuai
author_sort Cai, Congbo
collection PubMed
description Background: Cuproptosis is a novel cell death mechanism, and FDX1 is a key gene associated with cuproptosis. However, it is unclear whether FDX1 has prognostic and immunotherapeutic value for clear cell renal carcinoma (ccRCC). Methods: Data on FDX1 expression in ccRCC were extracted from various databases and validated using qRT-PCR and western blotting. Moreover, the survival prognosis, clinical features, methylation, and biological functions of FDX1 were evaluated, and the tumor immune dysfunction and exclusion (TIDE) score was used to explore the immunotherapy response to FDX1 in ccRCC. Results: The expression of FDX1 in ccRCC tissues was significantly lower than that in normal tissues, as validated by qRT-PCR and western blotting of patient samples (P < 0.01). Moreover, low FDX1 expression was related to shorter survival time and high immune activation, as indicated by alterations in the tumor mutational burden and tumor microenvironment, stronger immune cell infiltration and immunosuppression point expression, and a higher TIDE score. Conclusions: FDX1 could serve as a novel and accessible biomarker for predicting survival prognosis, tumor immune landscape, and immune responses in ccRCC.
format Online
Article
Text
id pubmed-10373983
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-103739832023-07-28 Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting Cai, Congbo Zhou, Kena Jing, Jing Ren, Yu Weng, Guobin Cen, Dong Wang, Xue Huang, Shuaishuai Aging (Albany NY) Research Paper Background: Cuproptosis is a novel cell death mechanism, and FDX1 is a key gene associated with cuproptosis. However, it is unclear whether FDX1 has prognostic and immunotherapeutic value for clear cell renal carcinoma (ccRCC). Methods: Data on FDX1 expression in ccRCC were extracted from various databases and validated using qRT-PCR and western blotting. Moreover, the survival prognosis, clinical features, methylation, and biological functions of FDX1 were evaluated, and the tumor immune dysfunction and exclusion (TIDE) score was used to explore the immunotherapy response to FDX1 in ccRCC. Results: The expression of FDX1 in ccRCC tissues was significantly lower than that in normal tissues, as validated by qRT-PCR and western blotting of patient samples (P < 0.01). Moreover, low FDX1 expression was related to shorter survival time and high immune activation, as indicated by alterations in the tumor mutational burden and tumor microenvironment, stronger immune cell infiltration and immunosuppression point expression, and a higher TIDE score. Conclusions: FDX1 could serve as a novel and accessible biomarker for predicting survival prognosis, tumor immune landscape, and immune responses in ccRCC. Impact Journals 2023-07-10 /pmc/articles/PMC10373983/ /pubmed/37432054 http://dx.doi.org/10.18632/aging.204807 Text en Copyright: © 2023 Cai et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Cai, Congbo
Zhou, Kena
Jing, Jing
Ren, Yu
Weng, Guobin
Cen, Dong
Wang, Xue
Huang, Shuaishuai
Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting
title Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting
title_full Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting
title_fullStr Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting
title_full_unstemmed Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting
title_short Confirmation of the predictive function of cuproptosis-related gene FDX1 in clear cell renal carcinoma using qRT-PCR and western blotting
title_sort confirmation of the predictive function of cuproptosis-related gene fdx1 in clear cell renal carcinoma using qrt-pcr and western blotting
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373983/
https://www.ncbi.nlm.nih.gov/pubmed/37432054
http://dx.doi.org/10.18632/aging.204807
work_keys_str_mv AT caicongbo confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT zhoukena confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT jingjing confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT renyu confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT wengguobin confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT cendong confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT wangxue confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting
AT huangshuaishuai confirmationofthepredictivefunctionofcuproptosisrelatedgenefdx1inclearcellrenalcarcinomausingqrtpcrandwesternblotting