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Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374021/ https://www.ncbi.nlm.nih.gov/pubmed/37520440 http://dx.doi.org/10.3389/fcimb.2023.1056866 |
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author | Hirano, Rika Nishita, Izumi Nakai, Riho Bito, Ayaka Sasabe, Ryunosuke Kurihara, Shin |
author_facet | Hirano, Rika Nishita, Izumi Nakai, Riho Bito, Ayaka Sasabe, Ryunosuke Kurihara, Shin |
author_sort | Hirano, Rika |
collection | PubMed |
description | In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silico analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota. |
format | Online Article Text |
id | pubmed-10374021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103740212023-07-28 Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria Hirano, Rika Nishita, Izumi Nakai, Riho Bito, Ayaka Sasabe, Ryunosuke Kurihara, Shin Front Cell Infect Microbiol Cellular and Infection Microbiology In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silico analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota. Frontiers Media S.A. 2023-07-13 /pmc/articles/PMC10374021/ /pubmed/37520440 http://dx.doi.org/10.3389/fcimb.2023.1056866 Text en Copyright © 2023 Hirano, Nishita, Nakai, Bito, Sasabe and Kurihara https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Hirano, Rika Nishita, Izumi Nakai, Riho Bito, Ayaka Sasabe, Ryunosuke Kurihara, Shin Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
title | Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
title_full | Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
title_fullStr | Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
title_full_unstemmed | Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
title_short | Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
title_sort | development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374021/ https://www.ncbi.nlm.nih.gov/pubmed/37520440 http://dx.doi.org/10.3389/fcimb.2023.1056866 |
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