Cargando…

Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria

In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silic...

Descripción completa

Detalles Bibliográficos
Autores principales: Hirano, Rika, Nishita, Izumi, Nakai, Riho, Bito, Ayaka, Sasabe, Ryunosuke, Kurihara, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374021/
https://www.ncbi.nlm.nih.gov/pubmed/37520440
http://dx.doi.org/10.3389/fcimb.2023.1056866
_version_ 1785078684207546368
author Hirano, Rika
Nishita, Izumi
Nakai, Riho
Bito, Ayaka
Sasabe, Ryunosuke
Kurihara, Shin
author_facet Hirano, Rika
Nishita, Izumi
Nakai, Riho
Bito, Ayaka
Sasabe, Ryunosuke
Kurihara, Shin
author_sort Hirano, Rika
collection PubMed
description In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silico analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota.
format Online
Article
Text
id pubmed-10374021
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-103740212023-07-28 Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria Hirano, Rika Nishita, Izumi Nakai, Riho Bito, Ayaka Sasabe, Ryunosuke Kurihara, Shin Front Cell Infect Microbiol Cellular and Infection Microbiology In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silico analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota. Frontiers Media S.A. 2023-07-13 /pmc/articles/PMC10374021/ /pubmed/37520440 http://dx.doi.org/10.3389/fcimb.2023.1056866 Text en Copyright © 2023 Hirano, Nishita, Nakai, Bito, Sasabe and Kurihara https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Hirano, Rika
Nishita, Izumi
Nakai, Riho
Bito, Ayaka
Sasabe, Ryunosuke
Kurihara, Shin
Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
title Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
title_full Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
title_fullStr Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
title_full_unstemmed Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
title_short Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
title_sort development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374021/
https://www.ncbi.nlm.nih.gov/pubmed/37520440
http://dx.doi.org/10.3389/fcimb.2023.1056866
work_keys_str_mv AT hiranorika developmentofculturemethodscapableofculturingawiderangeofpredominantspeciesofintestinalbacteria
AT nishitaizumi developmentofculturemethodscapableofculturingawiderangeofpredominantspeciesofintestinalbacteria
AT nakairiho developmentofculturemethodscapableofculturingawiderangeofpredominantspeciesofintestinalbacteria
AT bitoayaka developmentofculturemethodscapableofculturingawiderangeofpredominantspeciesofintestinalbacteria
AT sasaberyunosuke developmentofculturemethodscapableofculturingawiderangeofpredominantspeciesofintestinalbacteria
AT kuriharashin developmentofculturemethodscapableofculturingawiderangeofpredominantspeciesofintestinalbacteria