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In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
Differentiation of naive CD4(+) T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4(+) T cells can produce hep...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374024/ https://www.ncbi.nlm.nih.gov/pubmed/37520551 http://dx.doi.org/10.3389/fimmu.2023.1210836 |
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author | Ford, Shayne Lavondua Buus, Terkild Brink Nastasi, Claudia Geisler, Carsten Bonefeld, Charlotte Menné Ødum, Niels Woetmann, Anders |
author_facet | Ford, Shayne Lavondua Buus, Terkild Brink Nastasi, Claudia Geisler, Carsten Bonefeld, Charlotte Menné Ødum, Niels Woetmann, Anders |
author_sort | Ford, Shayne Lavondua |
collection | PubMed |
description | Differentiation of naive CD4(+) T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4(+) T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present. |
format | Online Article Text |
id | pubmed-10374024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103740242023-07-28 In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor Ford, Shayne Lavondua Buus, Terkild Brink Nastasi, Claudia Geisler, Carsten Bonefeld, Charlotte Menné Ødum, Niels Woetmann, Anders Front Immunol Immunology Differentiation of naive CD4(+) T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4(+) T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present. Frontiers Media S.A. 2023-07-13 /pmc/articles/PMC10374024/ /pubmed/37520551 http://dx.doi.org/10.3389/fimmu.2023.1210836 Text en Copyright © 2023 Ford, Buus, Nastasi, Geisler, Bonefeld, Ødum and Woetmann https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Ford, Shayne Lavondua Buus, Terkild Brink Nastasi, Claudia Geisler, Carsten Bonefeld, Charlotte Menné Ødum, Niels Woetmann, Anders In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor |
title |
In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor |
title_full |
In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor |
title_fullStr |
In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor |
title_full_unstemmed |
In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor |
title_short |
In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor |
title_sort | in vitro differentiated human cd4(+) t cells produce hepatocyte growth factor |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374024/ https://www.ncbi.nlm.nih.gov/pubmed/37520551 http://dx.doi.org/10.3389/fimmu.2023.1210836 |
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