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In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor

Differentiation of naive CD4(+) T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4(+) T cells can produce hep...

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Autores principales: Ford, Shayne Lavondua, Buus, Terkild Brink, Nastasi, Claudia, Geisler, Carsten, Bonefeld, Charlotte Menné, Ødum, Niels, Woetmann, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374024/
https://www.ncbi.nlm.nih.gov/pubmed/37520551
http://dx.doi.org/10.3389/fimmu.2023.1210836
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author Ford, Shayne Lavondua
Buus, Terkild Brink
Nastasi, Claudia
Geisler, Carsten
Bonefeld, Charlotte Menné
Ødum, Niels
Woetmann, Anders
author_facet Ford, Shayne Lavondua
Buus, Terkild Brink
Nastasi, Claudia
Geisler, Carsten
Bonefeld, Charlotte Menné
Ødum, Niels
Woetmann, Anders
author_sort Ford, Shayne Lavondua
collection PubMed
description Differentiation of naive CD4(+) T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4(+) T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present.
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spelling pubmed-103740242023-07-28 In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor Ford, Shayne Lavondua Buus, Terkild Brink Nastasi, Claudia Geisler, Carsten Bonefeld, Charlotte Menné Ødum, Niels Woetmann, Anders Front Immunol Immunology Differentiation of naive CD4(+) T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4(+) T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present. Frontiers Media S.A. 2023-07-13 /pmc/articles/PMC10374024/ /pubmed/37520551 http://dx.doi.org/10.3389/fimmu.2023.1210836 Text en Copyright © 2023 Ford, Buus, Nastasi, Geisler, Bonefeld, Ødum and Woetmann https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ford, Shayne Lavondua
Buus, Terkild Brink
Nastasi, Claudia
Geisler, Carsten
Bonefeld, Charlotte Menné
Ødum, Niels
Woetmann, Anders
In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
title In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
title_full In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
title_fullStr In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
title_full_unstemmed In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
title_short In vitro differentiated human CD4(+) T cells produce hepatocyte growth factor
title_sort in vitro differentiated human cd4(+) t cells produce hepatocyte growth factor
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374024/
https://www.ncbi.nlm.nih.gov/pubmed/37520551
http://dx.doi.org/10.3389/fimmu.2023.1210836
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