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Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications

Probiotics are defined as live organisms in the host that contribute to health benefits. Lactobacillus gasseri LM1065, isolated from human breast milk, was investigated for its probiotic properties based on its genome. Draft genome map and de novo assembly were performed using the PacBio RS II syste...

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Autores principales: Bae, Won-Young, Lee, Young Jin, Jung, Woo-Hyun, Shin, So Lim, Kim, Tae-Rahk, Sohn, Minn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374649/
https://www.ncbi.nlm.nih.gov/pubmed/37500806
http://dx.doi.org/10.1038/s41598-023-39454-2
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author Bae, Won-Young
Lee, Young Jin
Jung, Woo-Hyun
Shin, So Lim
Kim, Tae-Rahk
Sohn, Minn
author_facet Bae, Won-Young
Lee, Young Jin
Jung, Woo-Hyun
Shin, So Lim
Kim, Tae-Rahk
Sohn, Minn
author_sort Bae, Won-Young
collection PubMed
description Probiotics are defined as live organisms in the host that contribute to health benefits. Lactobacillus gasseri LM1065, isolated from human breast milk, was investigated for its probiotic properties based on its genome. Draft genome map and de novo assembly were performed using the PacBio RS II system and hierarchical genome assembly process (HGAP). Probiotic properties were determined by the resistance to gastric conditions, adherence ability, enzyme production, safety assessment and mobile genetic elements. The fungistatic effect and inhibition of hyphae transition were studied using the cell-free supernatant (CFS). L. gasseri LM1065 showed high gastric pepsin tolerance and mild tolerance to bile salts. Auto-aggregation and hydrophobicity were measured to be 61.21% and 61.55%, respectively. The adherence to the human intestinal epithelial cells was measured to be 2.02%. Antibiotic-resistance genes and putative virulence genes were not predicted in the genomic analysis, and antibiotic susceptibility was satisfied by the criteria of the European Food Safety Authority. CFS showed a fungistatic effect and suppressed the tricarboxylic acid cycle in Candida albicans (29.02%). CFS also inhibited the transition to true hyphae and damaged the blastoconidia. This study demonstrates the essential properties of this novel probiotic, L. gasseri LM1065, and potential to inhibit vaginal C. albicans infection.
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spelling pubmed-103746492023-07-29 Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications Bae, Won-Young Lee, Young Jin Jung, Woo-Hyun Shin, So Lim Kim, Tae-Rahk Sohn, Minn Sci Rep Article Probiotics are defined as live organisms in the host that contribute to health benefits. Lactobacillus gasseri LM1065, isolated from human breast milk, was investigated for its probiotic properties based on its genome. Draft genome map and de novo assembly were performed using the PacBio RS II system and hierarchical genome assembly process (HGAP). Probiotic properties were determined by the resistance to gastric conditions, adherence ability, enzyme production, safety assessment and mobile genetic elements. The fungistatic effect and inhibition of hyphae transition were studied using the cell-free supernatant (CFS). L. gasseri LM1065 showed high gastric pepsin tolerance and mild tolerance to bile salts. Auto-aggregation and hydrophobicity were measured to be 61.21% and 61.55%, respectively. The adherence to the human intestinal epithelial cells was measured to be 2.02%. Antibiotic-resistance genes and putative virulence genes were not predicted in the genomic analysis, and antibiotic susceptibility was satisfied by the criteria of the European Food Safety Authority. CFS showed a fungistatic effect and suppressed the tricarboxylic acid cycle in Candida albicans (29.02%). CFS also inhibited the transition to true hyphae and damaged the blastoconidia. This study demonstrates the essential properties of this novel probiotic, L. gasseri LM1065, and potential to inhibit vaginal C. albicans infection. Nature Publishing Group UK 2023-07-27 /pmc/articles/PMC10374649/ /pubmed/37500806 http://dx.doi.org/10.1038/s41598-023-39454-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bae, Won-Young
Lee, Young Jin
Jung, Woo-Hyun
Shin, So Lim
Kim, Tae-Rahk
Sohn, Minn
Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications
title Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications
title_full Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications
title_fullStr Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications
title_full_unstemmed Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications
title_short Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications
title_sort draft genome sequence and probiotic functional property analysis of lactobacillus gasseri lm1065 for food industry applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374649/
https://www.ncbi.nlm.nih.gov/pubmed/37500806
http://dx.doi.org/10.1038/s41598-023-39454-2
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