A protocol for visualizing active cathepsin K in osteoclasts with a quenched-fluorescence-activity-based probe

Herein, we provide a protocol for visualizing active osteoclast cathepsin K (CatK) with the quenched-fluorescent-activity-based probe qTJK17. We describe steps for isolating peripheral blood mononuclear cells, their differentiation into osteoclasts, and TRAP staining using an acid phosphatase leukoc...

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Detalles Bibliográficos
Autores principales: Korba, Aleksandra, Ciastoń, Izabela, Kozieł, Joanna, Kasperkiewicz, Paulina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374869/
https://www.ncbi.nlm.nih.gov/pubmed/37481728
http://dx.doi.org/10.1016/j.xpro.2023.102465

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