Complete sequences of conjugal helper plasmids pRK2013 and pEVS104

We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to...

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Detalles Bibliográficos
Autores principales: Hall, Ashley, Donohue, Timothy, Peters, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375283/
https://www.ncbi.nlm.nih.gov/pubmed/37521139
http://dx.doi.org/10.17912/micropub.biology.000882
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author Hall, Ashley
Donohue, Timothy
Peters, Jason
author_facet Hall, Ashley
Donohue, Timothy
Peters, Jason
author_sort Hall, Ashley
collection PubMed
description We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to aid future engineering efforts.
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spelling pubmed-103752832023-07-29 Complete sequences of conjugal helper plasmids pRK2013 and pEVS104 Hall, Ashley Donohue, Timothy Peters, Jason MicroPubl Biol Materials and Reagents We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to aid future engineering efforts. Caltech Library 2023-07-13 /pmc/articles/PMC10375283/ /pubmed/37521139 http://dx.doi.org/10.17912/micropub.biology.000882 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Hall, Ashley
Donohue, Timothy
Peters, Jason
Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
title Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
title_full Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
title_fullStr Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
title_full_unstemmed Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
title_short Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
title_sort complete sequences of conjugal helper plasmids prk2013 and pevs104
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375283/
https://www.ncbi.nlm.nih.gov/pubmed/37521139
http://dx.doi.org/10.17912/micropub.biology.000882
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