Cargando…

Complete sequences of conjugal helper plasmids pRK2013 and pEVS104

We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hall, Ashley, Donohue, Timothy, Peters, Jason
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2023
Materias:	Materials and Reagents
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375283/ https://www.ncbi.nlm.nih.gov/pubmed/37521139 http://dx.doi.org/10.17912/micropub.biology.000882

Ejemplares similares

mScarlet and split fluorophore mScarlet resources for plasmid-based CRISPR/Cas9 knock-in in C. elegans
por: Witten, Gillian, et al.
Publicado: (2023)

Generation and validation of pX-UASTattB for dose-dependent misexpression studies in Drosophila
por: Singh, Monika, et al.
Publicado: (2022)

Efficient generation of a single-copy eft-3p::TIR1::F2A:: BFP::AID*::NLS allele in the C. elegans ttTi5605 insertion site through recombination-mediated cassette exchange
por: Vo, An A., et al.
Publicado: (2021)

Expression of a FRET-based ATP Biosensor in the C. elegans Intestine
por: Soto, Jose, et al.
Publicado: (2020)

Immunofluorescence reveals neuron-specific promoter activity in non-neuronal cells
por: Lear, Sierra K., et al.
Publicado: (2018)

Genome editing of Caenorhabditis briggsae using CRISPR/Cas9 co-conversion marker dpy-10
por: Cohen, Sarah M, et al.
Publicado: (2019)

Worm corpses affect quantification of dauer recovery
por: Gabaldon, Carolaing, et al.
Publicado: (2019)

Using HDR and a template to introduce an in-frame HA tag on the 3′ end of the Xenopus laevis gata2.L open reading frame
por: Piccinni, Maya, et al.
Publicado: (2019)

Phase-separated protein dynamics are affected by fluorescent tag choice
por: Uebel, Celja J, et al.
Publicado: (2019)

An antibody staining protocol variation for nematodes that adds heat-induced antigen retrieval (HIAR)
por: Loer, Curtis, et al.
Publicado: (2019)

Modified auxin improves the auxin-inducible degradation (AID) system for laid C. elegans embryos
por: Negishi, Takefumi, et al.
Publicado: (2019)

Crossing two sperm chromatin-localized mCherry transgenes into a single C. elegans strain boosts signal intensity without harming sperm function
por: Wong, Amanda C, et al.
Publicado: (2020)

Tracking of centriole inheritance in C. elegans
por: Erpf, Anna C., et al.
Publicado: (2020)

A new anterior pharyngeal region specific fluorescent co-transformation marker
por: Bhattacharya, Abhishek, et al.
Publicado: (2019)

DVC interneuron cGAL driver in Caenorhabditis elegans
por: Oh, Jun Young, et al.
Publicado: (2019)

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing
por: Benner, Leif, et al.
Publicado: (2018)

Simplified detection of a point mutation in C. elegans using tetra-primer ARMS-PCR
por: Sullenberger, Matthew T., et al.
Publicado: (2018)

Mapping results for a set of cGAL effectors and drivers
por: Walton, Sophie J., et al.
Publicado: (2017)

Extending the CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences
por: Ebbing, Annabel, et al.
Publicado: (2017)

Auxin does not affect a suite of morphological or behavioral phenotypes in two wild-type C. elegans strains
por: McDiarmid, Troy A, et al.
Publicado: (2020)

Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
por: Luhur, Arthur, et al.
Publicado: (2021)

Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
por: Tembo, Maiwase, et al.
Publicado: (2021)

Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans
por: Sloan, Dillon E., et al.
Publicado: (2021)

Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf
por: Li, Yinan, et al.
Publicado: (2021)

Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
por: Nonet, Michael
Publicado: (2021)

The proline-rich domain of MML-1 is biologically important but not required for localization to target promoters
por: Ceballos, Ainhoa, et al.
Publicado: (2021)

Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
por: Takemura, Masahiko, et al.
Publicado: (2021)

Visualization of a curated Oryza sativa L. CDPKs Protein-Protein Interaction Network (CDPK-OsPPIN)
por: Marques, Joana, et al.
Publicado: (2022)

Balancer-assisted outcrossing to remove unwanted background mutations
por: Dejima, Katsufumi, et al.
Publicado: (2022)

The Cbr-DPY-10(Arg92Cys) modification is a reliable co-conversion marker for CRISPR/Cas9 genome editing in Caenorhabditis briggsae
por: Toker, Itai Antoine, et al.
Publicado: (2022)

Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
por: Gallegos, Maria, et al.
Publicado: (2021)

Optimizing expression of a single copy transgene in C. elegans
por: Dour, Scott, et al.
Publicado: (2021)

A histamine-gated channel is an efficient negative selection marker for C. elegans transgenesis
por: El Mouridi, Sonia, et al.
Publicado: (2021)

Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans
por: Nonet, Michael
Publicado: (2021)

moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
por: Snoozy, Jennifer, et al.
Publicado: (2022)

Near-infrared nuclear markers for Drosophila imaging
por: Pan, Yuanwang, et al.
Publicado: (2022)

An endogenous mCherry-tagged COSA-1 as a crossover investigation tool in Caenorhabditis elegans
por: Ezechukwu, Chiemekam Samuel, et al.
Publicado: (2022)

Enteroendocrine cell expression of split-GAL4 drivers bearing regulatory sequences associated with panneuronally expressed genes in Drosophila melanogaster
por: Holsopple, Jessica M., et al.
Publicado: (2022)

A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling in C. elegans
por: Pani, Ariel M, et al.
Publicado: (2022)

An in vivo toolkit to visualize endogenous LAG-2/Delta and LIN-12/Notch signaling in C. elegans
por: Medwig-Kinney, Taylor N, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_03cjjfshs720i92hmia26cg3d2