Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.

Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, possibly as a protective measure against irreversible damage, su...

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Detalles Bibliográficos
Autores principales: Jain, Ishutesh, Tran, Phong T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375284/
https://www.ncbi.nlm.nih.gov/pubmed/37521138
http://dx.doi.org/10.17912/micropub.biology.000911
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author Jain, Ishutesh
Tran, Phong T.
author_facet Jain, Ishutesh
Tran, Phong T.
author_sort Jain, Ishutesh
collection PubMed
description Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, possibly as a protective measure against irreversible damage, such as DNA damage. To explore the link between prolonged mitosis and DNA damage, we develop a microscopy-based assay utilizing Rad52-GFP as a marker for mitotic DNA damage. Through this assay, we provide evidence that mutants with prolonged mitosis exhibit increased Rad52 puncta, indicating an elevation in endogenous DNA damage.
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spelling pubmed-103752842023-07-29 Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast. Jain, Ishutesh Tran, Phong T. MicroPubl Biol New Methods Mitosis is usually shorter than other phases of the cell cycle and maintains a consistent duration despite variations in cell size and spindle size. This suggests the existence of a compensatory mechanism that ensures a short duration, possibly as a protective measure against irreversible damage, such as DNA damage. To explore the link between prolonged mitosis and DNA damage, we develop a microscopy-based assay utilizing Rad52-GFP as a marker for mitotic DNA damage. Through this assay, we provide evidence that mutants with prolonged mitosis exhibit increased Rad52 puncta, indicating an elevation in endogenous DNA damage. Caltech Library 2023-07-13 /pmc/articles/PMC10375284/ /pubmed/37521138 http://dx.doi.org/10.17912/micropub.biology.000911 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Jain, Ishutesh
Tran, Phong T.
Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
title Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
title_full Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
title_fullStr Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
title_full_unstemmed Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
title_short Prolongation of mitosis is associated with enhanced endogenous DNA damage in fission yeast.
title_sort prolongation of mitosis is associated with enhanced endogenous dna damage in fission yeast.
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375284/
https://www.ncbi.nlm.nih.gov/pubmed/37521138
http://dx.doi.org/10.17912/micropub.biology.000911
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