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Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals

The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in...

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Autores principales: Reyes-Morales, Rodolfo, Segundo-Ibañez, Patricia, Flores-de Los Ángeles, César, Vizcarra-Ramos, David, Ibañez-Galeana, Damián Iñaki, Salas-Cuevas, Gabriela, Olvera-Serrano, Ángel, Pérez-Silva, Nancy Bibiana, Rocha-Rocha, Valeria Magali, El-Kassi, Elie Girgis, Escobedo-Straffon, Jorge, Contreras-Mioni, Laura, Rosas-Díaz, Marisol, Lopez-Martinez, Karla María, Arias-Matus, Carlos Eduardo, Bautista-Rodriguez, Elizabeth, Nolasco-Quiroga, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375439/
https://www.ncbi.nlm.nih.gov/pubmed/37522063
http://dx.doi.org/10.3892/etm.2023.12097
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author Reyes-Morales, Rodolfo
Segundo-Ibañez, Patricia
Flores-de Los Ángeles, César
Vizcarra-Ramos, David
Ibañez-Galeana, Damián Iñaki
Salas-Cuevas, Gabriela
Olvera-Serrano, Ángel
Pérez-Silva, Nancy Bibiana
Rocha-Rocha, Valeria Magali
El-Kassi, Elie Girgis
Escobedo-Straffon, Jorge
Contreras-Mioni, Laura
Rosas-Díaz, Marisol
Lopez-Martinez, Karla María
Arias-Matus, Carlos Eduardo
Bautista-Rodriguez, Elizabeth
Nolasco-Quiroga, Manuel
author_facet Reyes-Morales, Rodolfo
Segundo-Ibañez, Patricia
Flores-de Los Ángeles, César
Vizcarra-Ramos, David
Ibañez-Galeana, Damián Iñaki
Salas-Cuevas, Gabriela
Olvera-Serrano, Ángel
Pérez-Silva, Nancy Bibiana
Rocha-Rocha, Valeria Magali
El-Kassi, Elie Girgis
Escobedo-Straffon, Jorge
Contreras-Mioni, Laura
Rosas-Díaz, Marisol
Lopez-Martinez, Karla María
Arias-Matus, Carlos Eduardo
Bautista-Rodriguez, Elizabeth
Nolasco-Quiroga, Manuel
author_sort Reyes-Morales, Rodolfo
collection PubMed
description The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43% were symptomatic positive individuals, 25% were asymptomatic positive individuals and 32% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5% and a specificity of 97.6% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100% and a specificity of 97.6% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring.
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spelling pubmed-103754392023-07-29 Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals Reyes-Morales, Rodolfo Segundo-Ibañez, Patricia Flores-de Los Ángeles, César Vizcarra-Ramos, David Ibañez-Galeana, Damián Iñaki Salas-Cuevas, Gabriela Olvera-Serrano, Ángel Pérez-Silva, Nancy Bibiana Rocha-Rocha, Valeria Magali El-Kassi, Elie Girgis Escobedo-Straffon, Jorge Contreras-Mioni, Laura Rosas-Díaz, Marisol Lopez-Martinez, Karla María Arias-Matus, Carlos Eduardo Bautista-Rodriguez, Elizabeth Nolasco-Quiroga, Manuel Exp Ther Med Articles The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43% were symptomatic positive individuals, 25% were asymptomatic positive individuals and 32% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5% and a specificity of 97.6% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100% and a specificity of 97.6% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring. D.A. Spandidos 2023-07-06 /pmc/articles/PMC10375439/ /pubmed/37522063 http://dx.doi.org/10.3892/etm.2023.12097 Text en Copyright: © Reyes-Morales et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Reyes-Morales, Rodolfo
Segundo-Ibañez, Patricia
Flores-de Los Ángeles, César
Vizcarra-Ramos, David
Ibañez-Galeana, Damián Iñaki
Salas-Cuevas, Gabriela
Olvera-Serrano, Ángel
Pérez-Silva, Nancy Bibiana
Rocha-Rocha, Valeria Magali
El-Kassi, Elie Girgis
Escobedo-Straffon, Jorge
Contreras-Mioni, Laura
Rosas-Díaz, Marisol
Lopez-Martinez, Karla María
Arias-Matus, Carlos Eduardo
Bautista-Rodriguez, Elizabeth
Nolasco-Quiroga, Manuel
Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
title Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
title_full Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
title_fullStr Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
title_full_unstemmed Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
title_short Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
title_sort reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of sars‑cov‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375439/
https://www.ncbi.nlm.nih.gov/pubmed/37522063
http://dx.doi.org/10.3892/etm.2023.12097
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