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A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents
BACKGROUND: In vivo patch-clamp recording techniques provide access to the sub- and suprathreshold membrane potential dynamics of individual neurons during behavior. However, maintaining recording stability throughout behavior is a significant challenge, and while methods for head restraint are comm...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375832/ https://www.ncbi.nlm.nih.gov/pubmed/36871604 http://dx.doi.org/10.1016/j.jneumeth.2023.109827 |
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author | Dacre, Joshua Sánchez Rivera, Michelle Schiemann, Julia Currie, Stephen Ammer, Julian J. Duguid, Ian |
author_facet | Dacre, Joshua Sánchez Rivera, Michelle Schiemann, Julia Currie, Stephen Ammer, Julian J. Duguid, Ian |
author_sort | Dacre, Joshua |
collection | PubMed |
description | BACKGROUND: In vivo patch-clamp recording techniques provide access to the sub- and suprathreshold membrane potential dynamics of individual neurons during behavior. However, maintaining recording stability throughout behavior is a significant challenge, and while methods for head restraint are commonly used to enhance stability, behaviorally related brain movement relative to the skull can severely impact the success rate and duration of whole-cell patch-clamp recordings. NEW METHOD: We developed a low-cost, biocompatible, and 3D-printable cranial implant capable of locally stabilizing brain movement, while permitting equivalent access to the brain when compared to a conventional craniotomy. RESULTS: Experiments in head-restrained behaving mice demonstrate that the cranial implant can reliably reduce the amplitude and speed of brain displacements, significantly improving the success rate of recordings across repeated bouts of motor behavior. COMPARISON WITH EXISTING METHOD(S): Our solution offers an improvement on currently available strategies for brain stabilization. Due to its small size, the implant can be retrofitted to most in vivo electrophysiology recording setups, providing a low cost, easily implementable solution for increasing intracellular recording stability in vivo. CONCLUSIONS: By facilitating stable whole-cell patch-clamp recordings in vivo, biocompatible 3D printed implants should accelerate the investigation of single neuron computations underlying behavior. |
format | Online Article Text |
id | pubmed-10375832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier/North-Holland Biomedical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-103758322023-07-29 A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents Dacre, Joshua Sánchez Rivera, Michelle Schiemann, Julia Currie, Stephen Ammer, Julian J. Duguid, Ian J Neurosci Methods Article BACKGROUND: In vivo patch-clamp recording techniques provide access to the sub- and suprathreshold membrane potential dynamics of individual neurons during behavior. However, maintaining recording stability throughout behavior is a significant challenge, and while methods for head restraint are commonly used to enhance stability, behaviorally related brain movement relative to the skull can severely impact the success rate and duration of whole-cell patch-clamp recordings. NEW METHOD: We developed a low-cost, biocompatible, and 3D-printable cranial implant capable of locally stabilizing brain movement, while permitting equivalent access to the brain when compared to a conventional craniotomy. RESULTS: Experiments in head-restrained behaving mice demonstrate that the cranial implant can reliably reduce the amplitude and speed of brain displacements, significantly improving the success rate of recordings across repeated bouts of motor behavior. COMPARISON WITH EXISTING METHOD(S): Our solution offers an improvement on currently available strategies for brain stabilization. Due to its small size, the implant can be retrofitted to most in vivo electrophysiology recording setups, providing a low cost, easily implementable solution for increasing intracellular recording stability in vivo. CONCLUSIONS: By facilitating stable whole-cell patch-clamp recordings in vivo, biocompatible 3D printed implants should accelerate the investigation of single neuron computations underlying behavior. Elsevier/North-Holland Biomedical Press 2023-04-15 /pmc/articles/PMC10375832/ /pubmed/36871604 http://dx.doi.org/10.1016/j.jneumeth.2023.109827 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dacre, Joshua Sánchez Rivera, Michelle Schiemann, Julia Currie, Stephen Ammer, Julian J. Duguid, Ian A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
title | A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
title_full | A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
title_fullStr | A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
title_full_unstemmed | A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
title_short | A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
title_sort | cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375832/ https://www.ncbi.nlm.nih.gov/pubmed/36871604 http://dx.doi.org/10.1016/j.jneumeth.2023.109827 |
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