Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway

[Image: see text] Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of...

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Autores principales: Meng, Xin, Ganapathy, Srividya, van Roemburg, Lars, Post, Marco, Brinks, Daan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375888/
https://www.ncbi.nlm.nih.gov/pubmed/37520318
http://dx.doi.org/10.1021/acsphyschemau.3c00003
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author Meng, Xin
Ganapathy, Srividya
van Roemburg, Lars
Post, Marco
Brinks, Daan
author_facet Meng, Xin
Ganapathy, Srividya
van Roemburg, Lars
Post, Marco
Brinks, Daan
author_sort Meng, Xin
collection PubMed
description [Image: see text] Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a GEVI subclass known for their high voltage sensitivity and fast response kinetics. Here we review the essential aspects of microbial rhodopsin photocycles that are critical to understanding the mechanisms of voltage sensitivity in these proteins and link them to insights from efforts to create faster, brighter and more sensitive microbial rhodopsin-based GEVIs.
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spelling pubmed-103758882023-07-29 Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway Meng, Xin Ganapathy, Srividya van Roemburg, Lars Post, Marco Brinks, Daan ACS Phys Chem Au [Image: see text] Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a GEVI subclass known for their high voltage sensitivity and fast response kinetics. Here we review the essential aspects of microbial rhodopsin photocycles that are critical to understanding the mechanisms of voltage sensitivity in these proteins and link them to insights from efforts to create faster, brighter and more sensitive microbial rhodopsin-based GEVIs. American Chemical Society 2023-05-03 /pmc/articles/PMC10375888/ /pubmed/37520318 http://dx.doi.org/10.1021/acsphyschemau.3c00003 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Meng, Xin
Ganapathy, Srividya
van Roemburg, Lars
Post, Marco
Brinks, Daan
Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
title Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
title_full Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
title_fullStr Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
title_full_unstemmed Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
title_short Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
title_sort voltage imaging with engineered proton-pumping rhodopsins: insights from the proton transfer pathway
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10375888/
https://www.ncbi.nlm.nih.gov/pubmed/37520318
http://dx.doi.org/10.1021/acsphyschemau.3c00003
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AT postmarco voltageimagingwithengineeredprotonpumpingrhodopsinsinsightsfromtheprotontransferpathway
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