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Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma

Glioblastoma (GBM) is an aggressive, common brain cancer known to disrupt redox biology, affecting behavior and DNA integrity. Past research remains inconclusive. To further understand this, an investigation was conducted on physical training’s effects on behavior, redox balance, and genomic stabili...

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Autores principales: Marqueze, Luis F. B., Costa, Amanda K., Pedroso, Giulia S., Vasconcellos, Franciane F., Pilger, Bruna I., Kindermann, Schellen, Andrade, Vanessa M., Alves, Ana C. B., Nery, Tatyana, Silva, Aderbal A., Carvalhal, Stephanie R. S., Zazula, Matheus F., Naliwaiko, Katya, Fernandes, Luiz C., Radak, Zsolt, Pinho, Ricardo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376052/
https://www.ncbi.nlm.nih.gov/pubmed/37507883
http://dx.doi.org/10.3390/antiox12071343
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author Marqueze, Luis F. B.
Costa, Amanda K.
Pedroso, Giulia S.
Vasconcellos, Franciane F.
Pilger, Bruna I.
Kindermann, Schellen
Andrade, Vanessa M.
Alves, Ana C. B.
Nery, Tatyana
Silva, Aderbal A.
Carvalhal, Stephanie R. S.
Zazula, Matheus F.
Naliwaiko, Katya
Fernandes, Luiz C.
Radak, Zsolt
Pinho, Ricardo A.
author_facet Marqueze, Luis F. B.
Costa, Amanda K.
Pedroso, Giulia S.
Vasconcellos, Franciane F.
Pilger, Bruna I.
Kindermann, Schellen
Andrade, Vanessa M.
Alves, Ana C. B.
Nery, Tatyana
Silva, Aderbal A.
Carvalhal, Stephanie R. S.
Zazula, Matheus F.
Naliwaiko, Katya
Fernandes, Luiz C.
Radak, Zsolt
Pinho, Ricardo A.
author_sort Marqueze, Luis F. B.
collection PubMed
description Glioblastoma (GBM) is an aggressive, common brain cancer known to disrupt redox biology, affecting behavior and DNA integrity. Past research remains inconclusive. To further understand this, an investigation was conducted on physical training’s effects on behavior, redox balance, and genomic stability in GBMA models. Forty-seven male C57BL/6J mice, 60 days old, were divided into GBM and sham groups (n = 15, n = 10, respectively), which were further subdivided into trained (Str, Gtr; n = 10, n = 12) and untrained (Sut, Gut; n = 10, n = 15) subsets. The trained mice performed moderate aerobic exercises on a treadmill five to six times a week for a month while untrained mice remained in their enclosures. Behavior was evaluated using open-field and rotarod tests. Post training, the mice were euthanized and brain, liver, bone marrow, and blood samples were analyzed for redox and genomic instability markers. The results indicated increased latency values in the trained GBM (Gtr) group, suggesting a beneficial impact of exercise. Elevated reactive oxygen species in the parietal tissue of untrained GBM mice (Gut) were reduced post training. Moreover, Gtr mice exhibited lower tail intensity, indicating less genomic instability. Thus, exercise could serve as a promising supplemental GBM treatment, modulating redox parameters and reducing genomic instability.
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spelling pubmed-103760522023-07-29 Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma Marqueze, Luis F. B. Costa, Amanda K. Pedroso, Giulia S. Vasconcellos, Franciane F. Pilger, Bruna I. Kindermann, Schellen Andrade, Vanessa M. Alves, Ana C. B. Nery, Tatyana Silva, Aderbal A. Carvalhal, Stephanie R. S. Zazula, Matheus F. Naliwaiko, Katya Fernandes, Luiz C. Radak, Zsolt Pinho, Ricardo A. Antioxidants (Basel) Article Glioblastoma (GBM) is an aggressive, common brain cancer known to disrupt redox biology, affecting behavior and DNA integrity. Past research remains inconclusive. To further understand this, an investigation was conducted on physical training’s effects on behavior, redox balance, and genomic stability in GBMA models. Forty-seven male C57BL/6J mice, 60 days old, were divided into GBM and sham groups (n = 15, n = 10, respectively), which were further subdivided into trained (Str, Gtr; n = 10, n = 12) and untrained (Sut, Gut; n = 10, n = 15) subsets. The trained mice performed moderate aerobic exercises on a treadmill five to six times a week for a month while untrained mice remained in their enclosures. Behavior was evaluated using open-field and rotarod tests. Post training, the mice were euthanized and brain, liver, bone marrow, and blood samples were analyzed for redox and genomic instability markers. The results indicated increased latency values in the trained GBM (Gtr) group, suggesting a beneficial impact of exercise. Elevated reactive oxygen species in the parietal tissue of untrained GBM mice (Gut) were reduced post training. Moreover, Gtr mice exhibited lower tail intensity, indicating less genomic instability. Thus, exercise could serve as a promising supplemental GBM treatment, modulating redox parameters and reducing genomic instability. MDPI 2023-06-26 /pmc/articles/PMC10376052/ /pubmed/37507883 http://dx.doi.org/10.3390/antiox12071343 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marqueze, Luis F. B.
Costa, Amanda K.
Pedroso, Giulia S.
Vasconcellos, Franciane F.
Pilger, Bruna I.
Kindermann, Schellen
Andrade, Vanessa M.
Alves, Ana C. B.
Nery, Tatyana
Silva, Aderbal A.
Carvalhal, Stephanie R. S.
Zazula, Matheus F.
Naliwaiko, Katya
Fernandes, Luiz C.
Radak, Zsolt
Pinho, Ricardo A.
Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma
title Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma
title_full Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma
title_fullStr Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma
title_full_unstemmed Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma
title_short Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma
title_sort regulation of redox profile and genomic instability by physical exercise contributes to neuroprotection in mice with experimental glioblastoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376052/
https://www.ncbi.nlm.nih.gov/pubmed/37507883
http://dx.doi.org/10.3390/antiox12071343
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