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Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity

SIMPLE SUMMARY: The Asian giant soft-shelled turtle (Pelochelys cantorii), belonging to the order Testudines (family Trionychidae, genus Pelochelys), is one of the largest inland aquatic turtle species. However, due to excessive economic development, Pc. cantorii is critically endangered and rarely...

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Autores principales: Liu, Xiaoli, Liu, Haiyang, Wang, Yakun, Li, Mingzhi, Ji, Liqin, Wang, Kaikuo, Wei, Chengqing, Li, Wei, Chen, Chen, Yu, Lingyun, Zhu, Xinping, Hong, Xiaoyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376104/
https://www.ncbi.nlm.nih.gov/pubmed/37508370
http://dx.doi.org/10.3390/biology12070939
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author Liu, Xiaoli
Liu, Haiyang
Wang, Yakun
Li, Mingzhi
Ji, Liqin
Wang, Kaikuo
Wei, Chengqing
Li, Wei
Chen, Chen
Yu, Lingyun
Zhu, Xinping
Hong, Xiaoyou
author_facet Liu, Xiaoli
Liu, Haiyang
Wang, Yakun
Li, Mingzhi
Ji, Liqin
Wang, Kaikuo
Wei, Chengqing
Li, Wei
Chen, Chen
Yu, Lingyun
Zhu, Xinping
Hong, Xiaoyou
author_sort Liu, Xiaoli
collection PubMed
description SIMPLE SUMMARY: The Asian giant soft-shelled turtle (Pelochelys cantorii), belonging to the order Testudines (family Trionychidae, genus Pelochelys), is one of the largest inland aquatic turtle species. However, due to excessive economic development, Pc. cantorii is critically endangered and rarely seen in the wild. As early as 1989, China listed the turtle as a key aquatic wildlife protection animal at the national level, but the conservation biology of Pc. cantorii has not been fully elucidated due to a lack of reference genomes. Here, based on a high-quality chromosome-level genome for Pc. cantorii, acquired by a combination of Illumina short-read, PacBio long-read and Hi-C scaffolding technologies in a previous study, we analyzed the evolutionary state of Pc. cantorii. Moreover, we found that several candidate genes associated with tumor suppression, growth and age were expanded, implicating their potential roles in the exceptional longevity of turtles. These findings will be an enabling resource for genetic and genomic studies to support fundamental insights into Pc. cantorii conservation. ABSTRACT: The Asian giant soft-shelled turtle, Pelochelys cantorii (Trionychidae), is one of the largest aquatic turtles in China and was designated as a First-Grade Protected Animal in China in 1989. Previous investigation based on a combination of Illumina short-read, PacBio long-read and Hi-C scaffolding technologies acquired a high-quality chromosome-level genome of Pc. cantorii. In this study, comparative genomic analysis between Pc. cantorii and 16 other vertebrate genomes indicated that turtles separated from the ancestor of archosaurians approximately 256.6 (95% highest posterior density interval, 263.6–251.9) million years ago (Mya) (Upper Permian to Triassic) and that Pc. cantorii separated from the ancestor of Pd. sinensis and R. swinhoei approximately 59.3 (95% highest posterior density interval, 64.3–54.3) Mya. Moreover, several candidate genes, such as VWA5A, ABCG2, A2M and IGSF1, associated with tumor suppression, growth and age were expanded, implicating their potential roles in the exceptional longevity of turtles. This new chromosome-level assembly has important scientific value in the study of conservation of Pc. cantorii and also enriches the evolutionary investigation of turtle species.
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spelling pubmed-103761042023-07-29 Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity Liu, Xiaoli Liu, Haiyang Wang, Yakun Li, Mingzhi Ji, Liqin Wang, Kaikuo Wei, Chengqing Li, Wei Chen, Chen Yu, Lingyun Zhu, Xinping Hong, Xiaoyou Biology (Basel) Article SIMPLE SUMMARY: The Asian giant soft-shelled turtle (Pelochelys cantorii), belonging to the order Testudines (family Trionychidae, genus Pelochelys), is one of the largest inland aquatic turtle species. However, due to excessive economic development, Pc. cantorii is critically endangered and rarely seen in the wild. As early as 1989, China listed the turtle as a key aquatic wildlife protection animal at the national level, but the conservation biology of Pc. cantorii has not been fully elucidated due to a lack of reference genomes. Here, based on a high-quality chromosome-level genome for Pc. cantorii, acquired by a combination of Illumina short-read, PacBio long-read and Hi-C scaffolding technologies in a previous study, we analyzed the evolutionary state of Pc. cantorii. Moreover, we found that several candidate genes associated with tumor suppression, growth and age were expanded, implicating their potential roles in the exceptional longevity of turtles. These findings will be an enabling resource for genetic and genomic studies to support fundamental insights into Pc. cantorii conservation. ABSTRACT: The Asian giant soft-shelled turtle, Pelochelys cantorii (Trionychidae), is one of the largest aquatic turtles in China and was designated as a First-Grade Protected Animal in China in 1989. Previous investigation based on a combination of Illumina short-read, PacBio long-read and Hi-C scaffolding technologies acquired a high-quality chromosome-level genome of Pc. cantorii. In this study, comparative genomic analysis between Pc. cantorii and 16 other vertebrate genomes indicated that turtles separated from the ancestor of archosaurians approximately 256.6 (95% highest posterior density interval, 263.6–251.9) million years ago (Mya) (Upper Permian to Triassic) and that Pc. cantorii separated from the ancestor of Pd. sinensis and R. swinhoei approximately 59.3 (95% highest posterior density interval, 64.3–54.3) Mya. Moreover, several candidate genes, such as VWA5A, ABCG2, A2M and IGSF1, associated with tumor suppression, growth and age were expanded, implicating their potential roles in the exceptional longevity of turtles. This new chromosome-level assembly has important scientific value in the study of conservation of Pc. cantorii and also enriches the evolutionary investigation of turtle species. MDPI 2023-06-30 /pmc/articles/PMC10376104/ /pubmed/37508370 http://dx.doi.org/10.3390/biology12070939 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xiaoli
Liu, Haiyang
Wang, Yakun
Li, Mingzhi
Ji, Liqin
Wang, Kaikuo
Wei, Chengqing
Li, Wei
Chen, Chen
Yu, Lingyun
Zhu, Xinping
Hong, Xiaoyou
Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity
title Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity
title_full Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity
title_fullStr Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity
title_full_unstemmed Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity
title_short Chromosome-Level Analysis of the Pelochelys cantorii Genome Provides Insights to Its Immunity, Growth and Longevity
title_sort chromosome-level analysis of the pelochelys cantorii genome provides insights to its immunity, growth and longevity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376104/
https://www.ncbi.nlm.nih.gov/pubmed/37508370
http://dx.doi.org/10.3390/biology12070939
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