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Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles
Bacterial biofilms are a significant problem in the food industry, as they are difficult to eradicate and represent a threat to consumer health. Currently, nanoparticles as an alternative to traditional chemical disinfectants have garnered much attention due to their broad-spectrum antibacterial act...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376474/ https://www.ncbi.nlm.nih.gov/pubmed/37508180 http://dx.doi.org/10.3390/antibiotics12071084 |
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author | Verduzco-Chavira, Karen Vallejo-Cardona, Alba Adriana González-Garibay, Angélica Sofía Torres-González, Omar Ricardo Sánchez-Hernández, Iván Moisés Flores-Fernández, Jose Miguel Padilla-Camberos, Eduardo |
author_facet | Verduzco-Chavira, Karen Vallejo-Cardona, Alba Adriana González-Garibay, Angélica Sofía Torres-González, Omar Ricardo Sánchez-Hernández, Iván Moisés Flores-Fernández, Jose Miguel Padilla-Camberos, Eduardo |
author_sort | Verduzco-Chavira, Karen |
collection | PubMed |
description | Bacterial biofilms are a significant problem in the food industry, as they are difficult to eradicate and represent a threat to consumer health. Currently, nanoparticles as an alternative to traditional chemical disinfectants have garnered much attention due to their broad-spectrum antibacterial activity and low toxicity. In this study, silver nanoparticles (AgNPs) were synthesized by a biological method using a Jacaranda mimosifolia flower aqueous extract and by a chemical method, and the factors affecting both syntheses were optimized. The nanoparticles were characterized by Ultraviolet–visible (UV–Vis) spectrophotometry, Fourier-transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), X-ray diffraction (XRD), and Transmission electron microscopy (TEM) with a spherical and uniform shape. The antibacterial and antibiofilm formation activity was carried out on bacterial species of Pseudomonas aeruginosa and Staphylococcus aureus with the capacity to form biofilm. The minimum inhibitory concentration was 117.5 μg/mL for the chemical and 5.3 μg/mL for the biological nanoparticles. Both types of nanoparticles showed antibiofilm activity in the qualitative Congo red test and in the quantitative microplate test. Antibiofilm activity tests on fresh lettuce showed that biological nanoparticles decreased the population of S. aureus and P. aeruginosa by 0.63 and 2.38 logarithms, respectively, while chemical nanoparticles had little microbial reduction. In conclusion, the biologically synthesized nanoparticles showed greater antibiofilm activity. Therefore, these results suggest their potential application in the formulation of sanitizing products for the food and healthcare industries. |
format | Online Article Text |
id | pubmed-10376474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103764742023-07-29 Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles Verduzco-Chavira, Karen Vallejo-Cardona, Alba Adriana González-Garibay, Angélica Sofía Torres-González, Omar Ricardo Sánchez-Hernández, Iván Moisés Flores-Fernández, Jose Miguel Padilla-Camberos, Eduardo Antibiotics (Basel) Article Bacterial biofilms are a significant problem in the food industry, as they are difficult to eradicate and represent a threat to consumer health. Currently, nanoparticles as an alternative to traditional chemical disinfectants have garnered much attention due to their broad-spectrum antibacterial activity and low toxicity. In this study, silver nanoparticles (AgNPs) were synthesized by a biological method using a Jacaranda mimosifolia flower aqueous extract and by a chemical method, and the factors affecting both syntheses were optimized. The nanoparticles were characterized by Ultraviolet–visible (UV–Vis) spectrophotometry, Fourier-transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), X-ray diffraction (XRD), and Transmission electron microscopy (TEM) with a spherical and uniform shape. The antibacterial and antibiofilm formation activity was carried out on bacterial species of Pseudomonas aeruginosa and Staphylococcus aureus with the capacity to form biofilm. The minimum inhibitory concentration was 117.5 μg/mL for the chemical and 5.3 μg/mL for the biological nanoparticles. Both types of nanoparticles showed antibiofilm activity in the qualitative Congo red test and in the quantitative microplate test. Antibiofilm activity tests on fresh lettuce showed that biological nanoparticles decreased the population of S. aureus and P. aeruginosa by 0.63 and 2.38 logarithms, respectively, while chemical nanoparticles had little microbial reduction. In conclusion, the biologically synthesized nanoparticles showed greater antibiofilm activity. Therefore, these results suggest their potential application in the formulation of sanitizing products for the food and healthcare industries. MDPI 2023-06-21 /pmc/articles/PMC10376474/ /pubmed/37508180 http://dx.doi.org/10.3390/antibiotics12071084 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Verduzco-Chavira, Karen Vallejo-Cardona, Alba Adriana González-Garibay, Angélica Sofía Torres-González, Omar Ricardo Sánchez-Hernández, Iván Moisés Flores-Fernández, Jose Miguel Padilla-Camberos, Eduardo Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles |
title | Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles |
title_full | Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles |
title_fullStr | Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles |
title_full_unstemmed | Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles |
title_short | Antibacterial and Antibiofilm Activity of Chemically and Biologically Synthesized Silver Nanoparticles |
title_sort | antibacterial and antibiofilm activity of chemically and biologically synthesized silver nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376474/ https://www.ncbi.nlm.nih.gov/pubmed/37508180 http://dx.doi.org/10.3390/antibiotics12071084 |
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