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Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants

The actinomycete strain HSN-02 was isolated from the soil of a mining field in the Sandur region, Bellary, Karnataka, India. According to the morphological, cultural, physiological, and biochemical characteristics and the 16S rDNA sequence analysis, the strain HSN-02 was identified as Amycolatopsis...

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Autores principales: Math, Halaswamy Hire, Kumar, Raju Suresh, Chakraborty, Bidhayak, Almansour, Abdulrahman I., Perumal, Karthikeyan, Kantli, Girish Babu, Nayaka, Sreenivasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376496/
https://www.ncbi.nlm.nih.gov/pubmed/37508271
http://dx.doi.org/10.3390/antibiotics12071175
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author Math, Halaswamy Hire
Kumar, Raju Suresh
Chakraborty, Bidhayak
Almansour, Abdulrahman I.
Perumal, Karthikeyan
Kantli, Girish Babu
Nayaka, Sreenivasa
author_facet Math, Halaswamy Hire
Kumar, Raju Suresh
Chakraborty, Bidhayak
Almansour, Abdulrahman I.
Perumal, Karthikeyan
Kantli, Girish Babu
Nayaka, Sreenivasa
author_sort Math, Halaswamy Hire
collection PubMed
description The actinomycete strain HSN-02 was isolated from the soil of a mining field in the Sandur region, Bellary, Karnataka, India. According to the morphological, cultural, physiological, and biochemical characteristics and the 16S rDNA sequence analysis, the strain HSN-02 was identified as Amycolatopsis sp. The antimicrobial activity strain HSN-02 presented stable and moderate inhibitory activity against human pathogens. In pot experiments in the greenhouse, the development of Cercospora leaf spot was markedly suppressed by treatment with the purified compound from the strain HSN-02, and the control efficacy was 45.04 ± 1.30% in Septoria lycopersici-infected tomato plants. A prominent compound was obtained from the fermentation broth of the strain HSN-02 using column chromatography and HPLC. The chemical structural analyses using UV, FTIR, HR-ESI-MS, and NMR confirmed that the compound produced by the strain HSN-02 is 7-hydroxyflavone. This investigation showed the role which the actinomycete strain can play in controlling leaf spots caused by S. lycopersici to reduce treatments with chemical fungicides.
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spelling pubmed-103764962023-07-29 Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants Math, Halaswamy Hire Kumar, Raju Suresh Chakraborty, Bidhayak Almansour, Abdulrahman I. Perumal, Karthikeyan Kantli, Girish Babu Nayaka, Sreenivasa Antibiotics (Basel) Article The actinomycete strain HSN-02 was isolated from the soil of a mining field in the Sandur region, Bellary, Karnataka, India. According to the morphological, cultural, physiological, and biochemical characteristics and the 16S rDNA sequence analysis, the strain HSN-02 was identified as Amycolatopsis sp. The antimicrobial activity strain HSN-02 presented stable and moderate inhibitory activity against human pathogens. In pot experiments in the greenhouse, the development of Cercospora leaf spot was markedly suppressed by treatment with the purified compound from the strain HSN-02, and the control efficacy was 45.04 ± 1.30% in Septoria lycopersici-infected tomato plants. A prominent compound was obtained from the fermentation broth of the strain HSN-02 using column chromatography and HPLC. The chemical structural analyses using UV, FTIR, HR-ESI-MS, and NMR confirmed that the compound produced by the strain HSN-02 is 7-hydroxyflavone. This investigation showed the role which the actinomycete strain can play in controlling leaf spots caused by S. lycopersici to reduce treatments with chemical fungicides. MDPI 2023-07-11 /pmc/articles/PMC10376496/ /pubmed/37508271 http://dx.doi.org/10.3390/antibiotics12071175 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Math, Halaswamy Hire
Kumar, Raju Suresh
Chakraborty, Bidhayak
Almansour, Abdulrahman I.
Perumal, Karthikeyan
Kantli, Girish Babu
Nayaka, Sreenivasa
Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
title Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
title_full Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
title_fullStr Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
title_full_unstemmed Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
title_short Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants
title_sort antimicrobial efficacy of 7-hydroxyflavone derived from amycolatopsis sp. hsn-02 and its biocontrol potential on cercospora leaf spot disease in tomato plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376496/
https://www.ncbi.nlm.nih.gov/pubmed/37508271
http://dx.doi.org/10.3390/antibiotics12071175
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