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Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis
According to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376753/ https://www.ncbi.nlm.nih.gov/pubmed/37508297 http://dx.doi.org/10.3390/antibiotics12071201 |
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author | Eshboev, Farkhod Karakozova, Marina Abdurakhmanov, Jaloliddin Bobakulov, Khayrulla Dolimov, Khayotjon Abdurashidov, Akhror Baymirzaev, Asadali Makhnyov, Artyom Terenteva, Ekaterina Sasmakov, Sobirdjan Piyakina, Galina Egamberdieva, Dilfuza Nazarov, Pavel A. Azimova, Shakhnoz |
author_facet | Eshboev, Farkhod Karakozova, Marina Abdurakhmanov, Jaloliddin Bobakulov, Khayrulla Dolimov, Khayotjon Abdurashidov, Akhror Baymirzaev, Asadali Makhnyov, Artyom Terenteva, Ekaterina Sasmakov, Sobirdjan Piyakina, Galina Egamberdieva, Dilfuza Nazarov, Pavel A. Azimova, Shakhnoz |
author_sort | Eshboev, Farkhod |
collection | PubMed |
description | According to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation antibiotics. The antimicrobial and cytotoxic effects of ethyl acetate extracts of nine endophytic fungal isolates obtained from Hyssopus officinalis were investigated for bioassay-guided isolation of the natural compounds. An extract of isolate VII showed the highest antimicrobial activities against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus (30.12 ± 0.20 mm and 35.21 ± 0.20 mm) and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (30.41 ± 0.23 mm and 25.12 ± 0.25 mm) among the tested extracts of isolates. Molecular identification of isolate VII confirmed it as Chaetomium elatum based on sequencing of its ITS genes, and it was discovered that this was the first time C. elatum had been isolated from H. officinalis. This isolate was cultured at a large scale for the isolation and identification of the active compound. Penicillic acid was isolated for the first time from C. elatum and its chemical structure was established by NMR spectroscopy. The penicillic acid showed strong antibacterial activities against Bacillus subtilis and Staphylococcus aureus with 20.68 mm and 25.51 mm inhibition zones, respectively. In addition, MIC and MBC values and antibiofilm activities of penicillic acid were determined. It was found that penicillic acid reduced the level of biofilms in proportion to antibacterial activity. |
format | Online Article Text |
id | pubmed-10376753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103767532023-07-29 Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis Eshboev, Farkhod Karakozova, Marina Abdurakhmanov, Jaloliddin Bobakulov, Khayrulla Dolimov, Khayotjon Abdurashidov, Akhror Baymirzaev, Asadali Makhnyov, Artyom Terenteva, Ekaterina Sasmakov, Sobirdjan Piyakina, Galina Egamberdieva, Dilfuza Nazarov, Pavel A. Azimova, Shakhnoz Antibiotics (Basel) Article According to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation antibiotics. The antimicrobial and cytotoxic effects of ethyl acetate extracts of nine endophytic fungal isolates obtained from Hyssopus officinalis were investigated for bioassay-guided isolation of the natural compounds. An extract of isolate VII showed the highest antimicrobial activities against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus (30.12 ± 0.20 mm and 35.21 ± 0.20 mm) and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (30.41 ± 0.23 mm and 25.12 ± 0.25 mm) among the tested extracts of isolates. Molecular identification of isolate VII confirmed it as Chaetomium elatum based on sequencing of its ITS genes, and it was discovered that this was the first time C. elatum had been isolated from H. officinalis. This isolate was cultured at a large scale for the isolation and identification of the active compound. Penicillic acid was isolated for the first time from C. elatum and its chemical structure was established by NMR spectroscopy. The penicillic acid showed strong antibacterial activities against Bacillus subtilis and Staphylococcus aureus with 20.68 mm and 25.51 mm inhibition zones, respectively. In addition, MIC and MBC values and antibiofilm activities of penicillic acid were determined. It was found that penicillic acid reduced the level of biofilms in proportion to antibacterial activity. MDPI 2023-07-18 /pmc/articles/PMC10376753/ /pubmed/37508297 http://dx.doi.org/10.3390/antibiotics12071201 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eshboev, Farkhod Karakozova, Marina Abdurakhmanov, Jaloliddin Bobakulov, Khayrulla Dolimov, Khayotjon Abdurashidov, Akhror Baymirzaev, Asadali Makhnyov, Artyom Terenteva, Ekaterina Sasmakov, Sobirdjan Piyakina, Galina Egamberdieva, Dilfuza Nazarov, Pavel A. Azimova, Shakhnoz Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis |
title | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis |
title_full | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis |
title_fullStr | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis |
title_full_unstemmed | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis |
title_short | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis |
title_sort | antimicrobial and cytotoxic activities of the secondary metabolites of endophytic fungi isolated from the medicinal plant hyssopus officinalis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376753/ https://www.ncbi.nlm.nih.gov/pubmed/37508297 http://dx.doi.org/10.3390/antibiotics12071201 |
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