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The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome

Acute respiratory distress syndrome (ARDS) is one of the most severe complications of the COVID-19 disease. The role of IL-33 and calpain 1 was previously described in lung infections and lung tissue damage. Our study examined the association between serum calpain 1 activity and IL-33 concentration...

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Autores principales: Loinjak, Domagoj, Mihić, Damir, Smolić, Robert, Maričić, Lana, Šahinović, Ines, Smolić, Martina, Sikora, Renata, Loinjak, Sanja, Dinjar, Kristijan, Včev, Aleksandar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376760/
https://www.ncbi.nlm.nih.gov/pubmed/37509486
http://dx.doi.org/10.3390/biomedicines11071847
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author Loinjak, Domagoj
Mihić, Damir
Smolić, Robert
Maričić, Lana
Šahinović, Ines
Smolić, Martina
Sikora, Renata
Loinjak, Sanja
Dinjar, Kristijan
Včev, Aleksandar
author_facet Loinjak, Domagoj
Mihić, Damir
Smolić, Robert
Maričić, Lana
Šahinović, Ines
Smolić, Martina
Sikora, Renata
Loinjak, Sanja
Dinjar, Kristijan
Včev, Aleksandar
author_sort Loinjak, Domagoj
collection PubMed
description Acute respiratory distress syndrome (ARDS) is one of the most severe complications of the COVID-19 disease. The role of IL-33 and calpain 1 was previously described in lung infections and lung tissue damage. Our study examined the association between serum calpain 1 activity and IL-33 concentration in patients with COVID-19 ARDS. In the research, we included 80 subjects who had COVID-19 pneumonia and divided them into 2 groups: 40 subjects with ARDS and 40 subjects without ARDS. The basis of the research was the collection of subjects’ data and the sampling of peripheral venous blood. The concentration of IL-33 was determined by the ELISA method and the activity of calpain 1 by the fluorometry method. Our research showed elevated calpain 1 activity and IL-33 concentration in the serum of COVID-19 patients who developed ARDS compared to those who did not develop ARDS and a positive correlation between them was established. Further, a positive correlation was established between the examined parameters and the severity of the disease, proinflammatory markers, and the use of mechanical ventilation. These results indicate a possible association and role of calpain 1 and IL-33 with the development of ARDS in COVID-19 patients.
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spelling pubmed-103767602023-07-29 The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome Loinjak, Domagoj Mihić, Damir Smolić, Robert Maričić, Lana Šahinović, Ines Smolić, Martina Sikora, Renata Loinjak, Sanja Dinjar, Kristijan Včev, Aleksandar Biomedicines Article Acute respiratory distress syndrome (ARDS) is one of the most severe complications of the COVID-19 disease. The role of IL-33 and calpain 1 was previously described in lung infections and lung tissue damage. Our study examined the association between serum calpain 1 activity and IL-33 concentration in patients with COVID-19 ARDS. In the research, we included 80 subjects who had COVID-19 pneumonia and divided them into 2 groups: 40 subjects with ARDS and 40 subjects without ARDS. The basis of the research was the collection of subjects’ data and the sampling of peripheral venous blood. The concentration of IL-33 was determined by the ELISA method and the activity of calpain 1 by the fluorometry method. Our research showed elevated calpain 1 activity and IL-33 concentration in the serum of COVID-19 patients who developed ARDS compared to those who did not develop ARDS and a positive correlation between them was established. Further, a positive correlation was established between the examined parameters and the severity of the disease, proinflammatory markers, and the use of mechanical ventilation. These results indicate a possible association and role of calpain 1 and IL-33 with the development of ARDS in COVID-19 patients. MDPI 2023-06-27 /pmc/articles/PMC10376760/ /pubmed/37509486 http://dx.doi.org/10.3390/biomedicines11071847 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loinjak, Domagoj
Mihić, Damir
Smolić, Robert
Maričić, Lana
Šahinović, Ines
Smolić, Martina
Sikora, Renata
Loinjak, Sanja
Dinjar, Kristijan
Včev, Aleksandar
The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome
title The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome
title_full The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome
title_fullStr The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome
title_full_unstemmed The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome
title_short The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome
title_sort correlation of serum calpain 1 activity and concentrations of interleukin 33 in covid-19 acute respiratory distress syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376760/
https://www.ncbi.nlm.nih.gov/pubmed/37509486
http://dx.doi.org/10.3390/biomedicines11071847
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