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Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation

Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressu...

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Autores principales: Wang, Dongzhe, Charoensombut, Narintadeach, Kawabata, Kinyoshi, Kimura, Tsuyoshi, Kishida, Akio, Ushida, Takashi, Furukawa, Katsuko S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376797/
https://www.ncbi.nlm.nih.gov/pubmed/37508841
http://dx.doi.org/10.3390/bioengineering10070814
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author Wang, Dongzhe
Charoensombut, Narintadeach
Kawabata, Kinyoshi
Kimura, Tsuyoshi
Kishida, Akio
Ushida, Takashi
Furukawa, Katsuko S.
author_facet Wang, Dongzhe
Charoensombut, Narintadeach
Kawabata, Kinyoshi
Kimura, Tsuyoshi
Kishida, Akio
Ushida, Takashi
Furukawa, Katsuko S.
author_sort Wang, Dongzhe
collection PubMed
description Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressure (HP) method stands out due to its low cytotoxicity and superior ECM preservation compared to the traditional detergent methods. Conventionally, 980 MPa was utilized in HP decellularization, including the first successful implementation of uterine decellularization previously reported by our team. However, structural protein denaturation caused by exceeding pressure led to a limited regeneration outcome in our previous research. This factor urged the study on the effects of pressure conditions in HP methods on decellularized scaffolds. The authors, therefore, fabricated a decellularized uterine scaffold at varying pressure conditions and evaluated the scaffold qualities from the perspective of cell removal and ECM preservation. The results show that by using lower decellularization pressure conditions of 250 MPa, uterine tissue can be decellularized with more preserved structural protein and mechanical properties, which is considered to be promising for decellularized uterine scaffold fabrication applications.
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spelling pubmed-103767972023-07-29 Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation Wang, Dongzhe Charoensombut, Narintadeach Kawabata, Kinyoshi Kimura, Tsuyoshi Kishida, Akio Ushida, Takashi Furukawa, Katsuko S. Bioengineering (Basel) Article Uterine regeneration using decellularization scaffolds provides a novel treatment for uterine factor infertility. Decellularized scaffolds require maximal removal of cellular components and minimal damage to the extracellular matrix (ECM). Among many decellularization methods, the hydrostatic pressure (HP) method stands out due to its low cytotoxicity and superior ECM preservation compared to the traditional detergent methods. Conventionally, 980 MPa was utilized in HP decellularization, including the first successful implementation of uterine decellularization previously reported by our team. However, structural protein denaturation caused by exceeding pressure led to a limited regeneration outcome in our previous research. This factor urged the study on the effects of pressure conditions in HP methods on decellularized scaffolds. The authors, therefore, fabricated a decellularized uterine scaffold at varying pressure conditions and evaluated the scaffold qualities from the perspective of cell removal and ECM preservation. The results show that by using lower decellularization pressure conditions of 250 MPa, uterine tissue can be decellularized with more preserved structural protein and mechanical properties, which is considered to be promising for decellularized uterine scaffold fabrication applications. MDPI 2023-07-07 /pmc/articles/PMC10376797/ /pubmed/37508841 http://dx.doi.org/10.3390/bioengineering10070814 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Dongzhe
Charoensombut, Narintadeach
Kawabata, Kinyoshi
Kimura, Tsuyoshi
Kishida, Akio
Ushida, Takashi
Furukawa, Katsuko S.
Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
title Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
title_full Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
title_fullStr Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
title_full_unstemmed Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
title_short Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation
title_sort effect of pressure conditions in uterine decellularization using hydrostatic pressure on structural protein preservation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376797/
https://www.ncbi.nlm.nih.gov/pubmed/37508841
http://dx.doi.org/10.3390/bioengineering10070814
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