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Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)

SIMPLE SUMMARY: The development of novel protein sources plays an important role in improving the economic benefit of aquatic products. The Chlorella vulgaris (C. vulgaris) powder is a novel non-grain single-cell protein with a high reproductive rate, short growth cycle, strong environmental toleran...

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Autores principales: Yang, Linlin, Cai, Minglang, Zhong, Lei, Shi, Yong, Xie, Shouqi, Hu, Yi, Zhang, Junzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376889/
https://www.ncbi.nlm.nih.gov/pubmed/37508052
http://dx.doi.org/10.3390/ani13142274
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author Yang, Linlin
Cai, Minglang
Zhong, Lei
Shi, Yong
Xie, Shouqi
Hu, Yi
Zhang, Junzhi
author_facet Yang, Linlin
Cai, Minglang
Zhong, Lei
Shi, Yong
Xie, Shouqi
Hu, Yi
Zhang, Junzhi
author_sort Yang, Linlin
collection PubMed
description SIMPLE SUMMARY: The development of novel protein sources plays an important role in improving the economic benefit of aquatic products. The Chlorella vulgaris (C. vulgaris) powder is a novel non-grain single-cell protein with a high reproductive rate, short growth cycle, strong environmental tolerance and easy artificial cultivation. In this experiment, grass carps (initial weight: 20.13 ± 0.09 g) were fed diets by replacing 0% (SM), 25% (X25), 50% (X50), 75% (X75) and 100% (X100) of SM with C. vulgaris for 8 weeks. In conclusion, the C. vulgaris powder replacement of 50% soybean meal was recommended as feed for grass carp. However, the positive effects were apparently weakened when the soybean meal was replaced with high levels of C. vulgaris powder. ABSTRACT: Chlorella vulgaris (C. vulgaris) powder is a novel non-grain single-cell protein with enormous potential to be a protein source. However, it is poorly studied in aquatic animals. The purpose of the present study was to explore the optimum replacement ratio of C. vulgaris powder and the influence of the substitution of soybean meal with C. vulgaris on grass carp (Ctenopharyngodon idella) in terms of growth performance, intestinal integrity and the microbial community. Five isonitrogenous and isolipidic diets were formulated by replacing 0% (SM, containing 30% soybean meal), 25% (X25), 50% (X50), 75% (X75) and 100% (X100) soybean meal with C. vulgaris. The feeding trial period lasted 8 weeks. At the end of the experimental trial, the X50 group showed higher FW, WGR and PER than the SM group (p < 0.05). The feed conversion ratio (FCR) of the X50 group was significantly lower than that of the SM group (p < 0.05). The X50 group showed the highest value of the goblet cell number, intestinal amylase and trypsin activities when compared with the SM group (p < 0.05). Replacing 50% soybean meal with C. vulgaris improved the intestinal barrier integrity, as evidenced by upregulating zo-1, zo-2 and occluding transcript (p < 0.05), and alleviated oxidative stress by an increased SOD enzymatic activity and transcript level, probably mediated through the Nrf2-keap1 signaling pathway (p < 0.05). Meanwhile, the X50 group enhanced intestinal immunity, as manifested by increased ACP and LZM activities (p < 0.05), and downregulated the tlr-4, tlr-7, tlr-8 and il-6 through the tlr pathway (p < 0.05). The functionally predicting pathways related to the nitrate respiration and nitrogen respiration were observably activated in the X50 group (p < 0.05). The X50 group improved the biological barrier, as manifested by increased Firmicutes and Rhodobacter (p < 0.05). In conclusion, dietary C. vulgaris powder could promote the growth performance of grass carp by restoring intestinal morphology, increasing digestive enzyme activities, improving antioxidant properties and immunity and optimizing the microflora structure. A C. vulgaris powder replacement of 50% soybean meal was recommended as feed for grass carp.
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spelling pubmed-103768892023-07-29 Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella) Yang, Linlin Cai, Minglang Zhong, Lei Shi, Yong Xie, Shouqi Hu, Yi Zhang, Junzhi Animals (Basel) Article SIMPLE SUMMARY: The development of novel protein sources plays an important role in improving the economic benefit of aquatic products. The Chlorella vulgaris (C. vulgaris) powder is a novel non-grain single-cell protein with a high reproductive rate, short growth cycle, strong environmental tolerance and easy artificial cultivation. In this experiment, grass carps (initial weight: 20.13 ± 0.09 g) were fed diets by replacing 0% (SM), 25% (X25), 50% (X50), 75% (X75) and 100% (X100) of SM with C. vulgaris for 8 weeks. In conclusion, the C. vulgaris powder replacement of 50% soybean meal was recommended as feed for grass carp. However, the positive effects were apparently weakened when the soybean meal was replaced with high levels of C. vulgaris powder. ABSTRACT: Chlorella vulgaris (C. vulgaris) powder is a novel non-grain single-cell protein with enormous potential to be a protein source. However, it is poorly studied in aquatic animals. The purpose of the present study was to explore the optimum replacement ratio of C. vulgaris powder and the influence of the substitution of soybean meal with C. vulgaris on grass carp (Ctenopharyngodon idella) in terms of growth performance, intestinal integrity and the microbial community. Five isonitrogenous and isolipidic diets were formulated by replacing 0% (SM, containing 30% soybean meal), 25% (X25), 50% (X50), 75% (X75) and 100% (X100) soybean meal with C. vulgaris. The feeding trial period lasted 8 weeks. At the end of the experimental trial, the X50 group showed higher FW, WGR and PER than the SM group (p < 0.05). The feed conversion ratio (FCR) of the X50 group was significantly lower than that of the SM group (p < 0.05). The X50 group showed the highest value of the goblet cell number, intestinal amylase and trypsin activities when compared with the SM group (p < 0.05). Replacing 50% soybean meal with C. vulgaris improved the intestinal barrier integrity, as evidenced by upregulating zo-1, zo-2 and occluding transcript (p < 0.05), and alleviated oxidative stress by an increased SOD enzymatic activity and transcript level, probably mediated through the Nrf2-keap1 signaling pathway (p < 0.05). Meanwhile, the X50 group enhanced intestinal immunity, as manifested by increased ACP and LZM activities (p < 0.05), and downregulated the tlr-4, tlr-7, tlr-8 and il-6 through the tlr pathway (p < 0.05). The functionally predicting pathways related to the nitrate respiration and nitrogen respiration were observably activated in the X50 group (p < 0.05). The X50 group improved the biological barrier, as manifested by increased Firmicutes and Rhodobacter (p < 0.05). In conclusion, dietary C. vulgaris powder could promote the growth performance of grass carp by restoring intestinal morphology, increasing digestive enzyme activities, improving antioxidant properties and immunity and optimizing the microflora structure. A C. vulgaris powder replacement of 50% soybean meal was recommended as feed for grass carp. MDPI 2023-07-12 /pmc/articles/PMC10376889/ /pubmed/37508052 http://dx.doi.org/10.3390/ani13142274 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Linlin
Cai, Minglang
Zhong, Lei
Shi, Yong
Xie, Shouqi
Hu, Yi
Zhang, Junzhi
Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)
title Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)
title_full Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)
title_fullStr Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)
title_full_unstemmed Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)
title_short Effects of Replacing Soybean Meal Protein with Chlorella vulgaris Powder on the Growth and Intestinal Health of Grass Carp (Ctenopharyngodon idella)
title_sort effects of replacing soybean meal protein with chlorella vulgaris powder on the growth and intestinal health of grass carp (ctenopharyngodon idella)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376889/
https://www.ncbi.nlm.nih.gov/pubmed/37508052
http://dx.doi.org/10.3390/ani13142274
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