Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells

New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influ...

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Autores principales: Efimova, Anna S., Ustimova, Mariya A., Chmelyuk, Nelly S., Abakumov, Maxim A., Fedorov, Yury V., Fedorova, Olga A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377282/
https://www.ncbi.nlm.nih.gov/pubmed/37504132
http://dx.doi.org/10.3390/bios13070734
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author Efimova, Anna S.
Ustimova, Mariya A.
Chmelyuk, Nelly S.
Abakumov, Maxim A.
Fedorov, Yury V.
Fedorova, Olga A.
author_facet Efimova, Anna S.
Ustimova, Mariya A.
Chmelyuk, Nelly S.
Abakumov, Maxim A.
Fedorov, Yury V.
Fedorova, Olga A.
author_sort Efimova, Anna S.
collection PubMed
description New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells.
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spelling pubmed-103772822023-07-29 Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells Efimova, Anna S. Ustimova, Mariya A. Chmelyuk, Nelly S. Abakumov, Maxim A. Fedorov, Yury V. Fedorova, Olga A. Biosensors (Basel) Article New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells. MDPI 2023-07-15 /pmc/articles/PMC10377282/ /pubmed/37504132 http://dx.doi.org/10.3390/bios13070734 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Efimova, Anna S.
Ustimova, Mariya A.
Chmelyuk, Nelly S.
Abakumov, Maxim A.
Fedorov, Yury V.
Fedorova, Olga A.
Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
title Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
title_full Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
title_fullStr Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
title_full_unstemmed Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
title_short Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
title_sort specific fluorescent probes for imaging dna in cell-free solution and in mitochondria in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377282/
https://www.ncbi.nlm.nih.gov/pubmed/37504132
http://dx.doi.org/10.3390/bios13070734
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