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Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration

The regeneration of articular cartilage remains a serious problem in various pathological conditions such as osteoarthritis, due to the tissue’s low self-healing capacity. The latest therapeutic approaches focus on the construction of biomaterials that induce cartilage repair. This research describe...

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Autores principales: Mantsou, Aglaia, Papachristou, Eleni, Keramidas, Panagiotis, Lamprou, Paraskevas, Pitou, Maria, Papi, Rigini M., Dimitriou, Katerina, Aggeli, Amalia, Choli-Papadopoulou, Theodora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377373/
https://www.ncbi.nlm.nih.gov/pubmed/37509529
http://dx.doi.org/10.3390/biomedicines11071890
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author Mantsou, Aglaia
Papachristou, Eleni
Keramidas, Panagiotis
Lamprou, Paraskevas
Pitou, Maria
Papi, Rigini M.
Dimitriou, Katerina
Aggeli, Amalia
Choli-Papadopoulou, Theodora
author_facet Mantsou, Aglaia
Papachristou, Eleni
Keramidas, Panagiotis
Lamprou, Paraskevas
Pitou, Maria
Papi, Rigini M.
Dimitriou, Katerina
Aggeli, Amalia
Choli-Papadopoulou, Theodora
author_sort Mantsou, Aglaia
collection PubMed
description The regeneration of articular cartilage remains a serious problem in various pathological conditions such as osteoarthritis, due to the tissue’s low self-healing capacity. The latest therapeutic approaches focus on the construction of biomaterials that induce cartilage repair. This research describes the design, synthesis, and investigation of a safe, “smart”, fibrous scaffold containing a genetically incorporated active peptide for chondrogenic induction. While possessing specific sequences and the respective mechanical properties from natural fibrous proteins, the fibers also incorporate a Transforming Growth Factor-β1 (TGF-β1)-derived peptide (YYVGRKPK) that can promote chondrogenesis. The scaffold formed stable porous networks with shear-thinning properties at 37 °C, as shown by SEM imaging and rheological characterization, and were proven to be non-toxic to human dental pulp stem cells (hDPSCs). Its chondrogenic capacity was evidenced by a strong increase in the expression of specific chondrogenesis gene markers SOX9, COL2, ACAN, TGFBR1A, and TGFBR2 in cells cultured on “scaffold-TGFβ1” for 21 days and by increased phosphorylation of intracellular signaling proteins Smad-2 and Erk-1/2. Additionally, intense staining of glycosaminoglycans was observed in these cells. According to our results, “scaffold-TGFβ1” is proposed for clinical studies as a safe, injectable treatment for cartilage degeneration.
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spelling pubmed-103773732023-07-29 Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration Mantsou, Aglaia Papachristou, Eleni Keramidas, Panagiotis Lamprou, Paraskevas Pitou, Maria Papi, Rigini M. Dimitriou, Katerina Aggeli, Amalia Choli-Papadopoulou, Theodora Biomedicines Article The regeneration of articular cartilage remains a serious problem in various pathological conditions such as osteoarthritis, due to the tissue’s low self-healing capacity. The latest therapeutic approaches focus on the construction of biomaterials that induce cartilage repair. This research describes the design, synthesis, and investigation of a safe, “smart”, fibrous scaffold containing a genetically incorporated active peptide for chondrogenic induction. While possessing specific sequences and the respective mechanical properties from natural fibrous proteins, the fibers also incorporate a Transforming Growth Factor-β1 (TGF-β1)-derived peptide (YYVGRKPK) that can promote chondrogenesis. The scaffold formed stable porous networks with shear-thinning properties at 37 °C, as shown by SEM imaging and rheological characterization, and were proven to be non-toxic to human dental pulp stem cells (hDPSCs). Its chondrogenic capacity was evidenced by a strong increase in the expression of specific chondrogenesis gene markers SOX9, COL2, ACAN, TGFBR1A, and TGFBR2 in cells cultured on “scaffold-TGFβ1” for 21 days and by increased phosphorylation of intracellular signaling proteins Smad-2 and Erk-1/2. Additionally, intense staining of glycosaminoglycans was observed in these cells. According to our results, “scaffold-TGFβ1” is proposed for clinical studies as a safe, injectable treatment for cartilage degeneration. MDPI 2023-07-03 /pmc/articles/PMC10377373/ /pubmed/37509529 http://dx.doi.org/10.3390/biomedicines11071890 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mantsou, Aglaia
Papachristou, Eleni
Keramidas, Panagiotis
Lamprou, Paraskevas
Pitou, Maria
Papi, Rigini M.
Dimitriou, Katerina
Aggeli, Amalia
Choli-Papadopoulou, Theodora
Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
title Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
title_full Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
title_fullStr Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
title_full_unstemmed Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
title_short Fabrication of a Smart Fibrous Biomaterial That Harbors an Active TGF-β1 Peptide: A Promising Approach for Cartilage Regeneration
title_sort fabrication of a smart fibrous biomaterial that harbors an active tgf-β1 peptide: a promising approach for cartilage regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377373/
https://www.ncbi.nlm.nih.gov/pubmed/37509529
http://dx.doi.org/10.3390/biomedicines11071890
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